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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Medulloblastoma, Neuroblastoma, Retinoblastoma
  • Region 2p24.3 / 2q11.2
  • Label    
  • Product No. LPS 009 (10 tests)
    LPS 009-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • N-MYC, 2p24.3, Red
  • LAF4, 2q11.2, Green

The N-MYC Amplification probe consists of a red 140kb probe spanning the N-MYC (MYCN) gene region at 2p24.3. The LAF4 gene probe in green at 2q11 acts as a control for chromosome 2.

 

Probe information

The proto-oncogene N-MYC (MYCN), located on chromosome 2 at 2p24.3, is a transcription factor that plays a role in regulation of cell growth and proliferation.

It is mainly expressed in the developing nervous system and is critical during neural crest embryogenesis. Myelocytomatosis viral homologue (MYCN) becomes rapidly down-regulated as tissues become terminally differentiated and growth-arrested1. Over-expression of MYCN seems to block differentiation and increase cell proliferation. MYCN amplification is the most important unfavourable prognostic factor for neuroblastoma and is amplified in 25% of cases2. Neuroblastoma is a dominating group of neuron tumours and is one of the most common solid tumours in children3.

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References

  1. Thomas WD et al., Int J of Bioch and Cell Biol 2004;36(5):771-5
  2. Look et al., J Clin Oncol. 1991 Apr;9(4):581-91
  3. Heim and Mitelman, Cancer cytogenetics 2nd Ed. 1995

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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