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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest ALL, AML
  • Region 9p21.3
    11q23.3
  • Label    
  • Product No. RU-LPH 083 (100μl)
    RU-LPH 083-S (50μl)
  • Intended use For research use only, not for use in diagnostic procedures. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • MLL, 11q23.3, Green
  • MLLT3, 9p21.3, Red

The MLL probe, labelled in green, covers a 200kb region including the MLL (KMT2A) gene. The MLLT3 probe, labelled in red, consists of a 140kb clone that covers the 5’ end of the MLLT3 gene and two clones (167kb and 186kb) that flank this.

 

Probe information

Translocations involving chromosome 11q23 frequently occur in both acute myeloid leukaemia (AML) and acute lymphoblastic leukaemia (ALL). In most cases, the MLL gene is involved but translocation partners are extremely variable, with 79 direct translocation partners having been molecularly characterised so far.

The most common MLL rearrangement in both AML and ALL involves fusion of the MLL and AFF1 (AF4) genes via a t(4;11)(q21;q23) translocation. Three of the other more common translocations involve the MLLT3 (AF9) gene on chromosome 9, the MLLT1 (ENL) gene on chromosome 19 or the MLLT4 (AF6) gene on chromosome 6:

  1. MLL/MLLT3 (AF9) - t(9;11)(p22;q23)
  2. MLL/MLLT1 (ENL) - t(11;19)(q23;p13.3)
  3. MLL/MLLT4 (AF6) - t(6;11)(q27;q23)

We now have a range of Research Use Only (RUO) Translocation, Dual Fusion probes to allow specific detection of these MLL rearrangements.

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References

  1. Meyer et al., Leukemia 2013;27(11):2165-76

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.

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