The MDM2 amplification probe consists of a 146kb red probe spanning the MDM2 gene and neighbouring CPM gene, with a green centromeric probe for chromosome 12 provided as a control.
The MDM2 gene, located at 12q15, when overexpressed, enhances the tumourigenic potential of cells.
The oncogene product forms a tight complex with the p53 tumour suppressor protein and can inhibit p53 mediated transactivation, leading to escape from p53 regulated growth control1. The Murine Double Minute 2 (MDM2) gene has been shown to be amplified in around 7% of human tumours2.
Many tumours overexpress MDM2, the most common being soft tissue tumours (20%), osteosarcomas (16%) and oesophageal carcinomas (13%)3. The use of FISH in well differentiated liposarcoma has been a valuable diagnostic tool4,5, whilst small-molecule MDM2 inhibitors that could act as innovative therapeutic tools have also been identified6.
In our hands, CytoCell FISH probes, including the ROS1 Proximal and ROS1 Distal probes, have proven to be of the highest quality with bright, easy to interpret signals, thus providing confidence in our results. OGT's customer support is outstanding, as their staff are extremely knowledgeable and truly care about their customers and their customers’ needs.
Director of Cytogenetics, Carolinas Pathology Group, USA