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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Glioma, Colorectal cancer, Oesophageal cancer, Lung cancer, Sarcoma
  • Region 12q15
  • Label    
  • Product No. LPS 016 (10 tests)
    LPS 016-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • MDM2, 12q15, Red
  • D12Z1, 12p11.1-q11.1, Green

The MDM2 amplification probe consists of a 146kb red probe spanning the MDM2 gene and neighbouring CPM gene, with a green centromeric probe for chromosome 12 provided as a control.

 

Probe information

The MDM2 gene, located at 12q15, when overexpressed, enhances the tumourigenic potential of cells.

The oncogene product forms a tight complex with the p53 tumour suppressor protein and can inhibit p53 mediated transactivation, leading to escape from p53 regulated growth control1. The Murine Double Minute 2 (MDM2) gene has been shown to be amplified in around 7% of human tumours2.

Many tumours overexpress MDM2, the most common being soft tissue tumours (20%), osteosarcomas (16%) and oesophageal carcinomas (13%)3. The use of FISH in well differentiated liposarcoma has been a valuable diagnostic tool4,5, whilst small-molecule MDM2 inhibitors that could act as innovative therapeutic tools have also been identified6.

What our customers say

References

  1. Momanad J et al., Cell 1992;69(7):1237-45
  2. Forslund et al., Mol Cancer Res. 2008 Feb;6(2):205-11
  3. Momand J, Nuc Acid Res 1998;26(15):3453-9
  4. Weaver J et al., Modern Path 2008;21(8):943-9
  5. Sirvent N et al., Am J Surg Pathol 2007;31(10):1476-98
  6. Vassilev LT, Trends Mol Med 2007;13(1):23-31

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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