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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lymphoma
  • Region 18q21.31-q21.32
  • Label    
  • Product No. LPS 017 (10 tests)
    LPS 017-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • MALT1, 18q21.31-q21.32, Red
  • MALT1, 18q21.31-q21.32, Green

The MALT1 Breakapart probe consists of two green clones (132kb and 306kb) and two red clones (171kb and 228kb), which are positioned on each side of the MALT1 gene.

 

Probe information

Extranodal marginal zone B-cell lymphoma (MZBCL) of the Mucosa-Associated Lymphoid Tissue (MALT) type is characterised by two main translocations involving MALT1; t(11;18)(q21;q21.32) and t(14;18)(q32;q21.32)1. Other MALT1 associated translocations are rare1.

However, there are no MALT1 translocations in nodal or splenic lymphomas1. The t(11;18) BIRC3 (Baculoviral IAP repeat containing 3 protein2,3)/MALT1 translocation is present in 18-33% of cases of MALT lymphoma2 and the resulting fusion protein induces NF-κB (nuclear factor kappa-light-chain-enhancer of activated B-cells), resulting in transcriptional activation3. 18% of MALT lymphomas harbour the t(14;18) MALT1/IGH translocation4.

The genetic abnormalities in MALT lymphomas reflect their site of origin. The t(14;18) translocation was most frequently found in MALT1 lymphomas of the parotid gland, liver, skin, and ocular adnexa – sites rare for the t(11;18). However, the pulmonary and gastrointestinal MALT lymphomas show high frequencies of t(11;18) and absence of the t(14;18)1,4.

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References

  1. Murga PEM et al., Leukemia 2003;17(11):2225-9
  2. Auer IA et al., Ann Oncol 1997;8(10):979-85
  3. Lucas PC et al., J Biol Chem 2001;276(22):19012-9
  4. Streubel B et al., Blood 2003;101(6):2335-9

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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