First slide
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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lymphoma
  • Region 8q24.21
    14q32.33
  • Label    
  • Product No. LPS 035 (10 tests)
    LPS 035-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • IGH, 14q32.33, Green
  • MYC, 8q24.21, Red

The IGH/MYC product consists of probes, labelled in green, covering the Constant, J, D and Variable segments of the IGH gene, and MYC probes, labelled in red. The MYC probe mix contains four probes, two 197kb, 277kb probes positioned centromeric to the MYC gene and two 186kb, 297kb probes positioned telomeric to the MYC gene, including the D8S1644 marker.

 

Probe information

The cMYC/IGH translocation, t(8;14)(q24;q32), and the variant forms t(2;8)(p13;q24) and t(8;22)(q24;q11) are found in Burkitt's lymphoma and mature B-cell or Burkitt's type Acute Lymphoblastic Leukaemia (ALL)1.

The t(8;14) is the most common and is found in approximately 85% of patients with Burkitt's lymphoma2. While translocation breakpoints on chromosome 14 are clustered to a narrow region 5' to the intron enhancer of the immunoglobulin heavy chain, the breakpoints on chromosome 8 can occur more than 340kb upstream of MYC, with no preferential site3. The translocation brings MYC into close proximity to the IgH enhancer and results in the up-regulation of MYC4. Over expression of the transcription factor stimulates gene amplification resulting in uncontrolled cell proliferation, which usually occurs in the late event of tumour progression5.

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References

  1. Berger R, Bernheim A, Cancer Genet Cytogenet 1982;7(3):231-44
  2. Hoffman, Ronald (2009). Hematology : basic principles and practice (5th ed. ed.). Philadelphia, PA: ChurchillLiving stone/Elsevier. pp. 1304–1305
  3. Joos et al., Human Molecular Genetics 1992;1(8):625-32
  4. Erikson J et al., Proc Natl Acad Sci USA 1983;80(3):820-4
  5. Shou et al., PNAS 2000;97(1):228-33

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.

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