CytoCell IGH/MALT1 Translocation, Dual Fusion FISH Probe

Product summary

Technology FISH
Application Solid tumour
Areas of interest Lymphoma
Region 14q32.33
18q21.31-q21.32
Label
Product Code LPS 034 (10 tests)
LPS 034-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

IGH, 14q32.33, Green
MALT1, 18q21.31-q21.32, Red

The IGH/MALT1 product consists of probes, labelled in green, covering the Constant, J, D and Variable segments of the IGH gene, and MALT1 probes, labelled in red. The MALT1 probe mix contains four probes, two 171kb, 228kb probes positioned centromeric to the MALT1 gene and two 132kb, 306kb probes positioned telomeric to the MALT1 gene.

Probe information

The t(14;18)(q32;q21) involving the Immunoglobulin heavy chain locus (IGH) at 14q32 and the Mucosa Associated Lymphoid Tissue Lymphoma Translocation gene 1 (MALT1) at 18q21 is a recurrent abnormality in MALT lymphoma¹.

Extranodal marginal zone B-cell lymphoma of the mucosa-associated lymphoid tissue is listed as a distinct clinicopathologic entity. MALT lymphomas comprise 7.6% of all non-Hodgkin lymphoma's (NHLs) and represents 1 of the 6 most common NHLs². MALT lymphomas are characterised by a proliferation of neoplastic marginal zone-related cells that invade the epithelial structures to generate lymphoepithelial lesions and colonise reactive lymphoid follicles³. t(14;18)/IGH-MALT1- positive MALT lymphomas originate in sites such as the liver, skin, ocular adnexa, or salivary glands¹. The molecular characteristics of the t(14;18) resemble those found in the t(14;18)/IGH-BCL2 (follicular lymphoma) and t(11;14)/MALT1-IGH (mantle cell lymphoma), suggesting that these translocations could be generated by common pathomechanisms involving illegitimate V(D)J-mediated recombination of IGH as well as non-homologous end joining (NHEJ) or alternative NHEJ repair pathways¹.

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.