First slide
Cytocell Logo

Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lymphoma
  • Region 14q32.33
  • Label    
  • Product Code LPS 032 (10 tests)
    LPS 032-S (5 tests)
  • Regulatory Status In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.



Probe specification

  • IGHC, 14q32.33, Red
  • IGHV, 14q32.33, Green

The IGH product consists of a 176kb probe, labelled in red, covering the Constant region of the gene and a green probe, covering a 617kb region within the Variable segment of the gene.


Probe information

Rearrangements involving the IGH gene have been identified in numerous different tumour types. There are a number of stereotypical translocations involved in each of the diseases and more are being described regularly.

The t(8;14)(q24;q32), involving IGH and the MYC gene, is the most common rearrangement in Burkitt's lymphoma and occurs in around 85% of cases1. IGH rearrangements have also been noted in 50% of non-Hodgkin B-cell lymphomas (NHLs) and have been correlated to clinically relevant subgroups2. One study showed that the t(14;18)(q32;q21) (IGH-BCL2) was found in 88.1% of follicular lymphomas and the t(11;14)(q13;q32) (IGH-CCND1) in 85.7% of mantle cell lymphomas2. IGH rearrangements were also identified in 52.5% of diffuse large B-cell lymphomas (DLBCL)1.

IGH is commonly rearranged in lymphomas and has many different translocation partners. We have, therefore, designed a split probe set for IGH, which allows the detection of rearrangements of the IGH gene, regardless of partner gene or chromosome involved.

What our customers say


  1. Hoffman, Ronald (2009). Hematology : basic principles and practice (5th ed. ed.). Philadelphia, PA: Churchill Livingstone/Elsevier. pp. 1304–1305
  2. Bernicot et al., Cytogenet Genome Res. 2007;118(2-4):345-52

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

1. Heat Pretreatment Dark Blue
  • Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
  • Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
Play Icon

Solid tumour FFPE FISH protocol

Request a quote for this product

FISH resources & support