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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lymphoma
  • Region 14q32.33
    18q21.33-q22.1
  • Label    
  • Product No. LPS 033 (10 tests)
    LPS 033-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • IGH, 14q32.33, Green
  • BCL2, 18q21.33-q22.1, Red

The IGH/BCL2 product consists of probes, labelled in green, covering the Constant, J, D and Variable segments of the IGH gene, and four probes labelled in red, positioned centromeric and telomeric to the BCL2 gene.

 

Probe information

The IGH/BCL2 fusion gene is brought about by a t(14;18)(q32;q21) translocation and is found in over 88% of follicular lymphoma cases1,2.

The translocation is thought to be brought about by an error in the joining function of the IGH gene, mediated by the recent observation that both IGH and BCL2 are arranged next to each other in 3D space in the normal B lymphocytes2. The translocation breakpoint at the end of the Joining (J) segment, and the subsequent fusion of the BCL2 gene to this region, results in the BCL2 gene coming under the regulatory control of those processes normally involved in maintenance of IGH gene activity3

The BCL2 gene itself has been shown to be involved in the regulation of apoptosis4.

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References

  1. Bernicot et al., Cytogenet Genome Res. 2007;118(2-4):345-52
  2. Roix et al., Nature Genetics 2003;34(3):287-91
  3. Stoos-Veić et al., Coll Antropol. 2010 Jun;34(2):425-9
  4. Sharpe et al., Biochim Biophys Acta. 20041644(2-3):107-13

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.

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