CytoCell HER2 (ERBB2) Amplification FISH Probe

Product summary

Technology FISH
Application Solid tumour
Areas of interest Breast cancer
Region 17q12
Label
Product Code LPS 001 (10 tests)
LPS 001-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

HER2 (ERBB2), 17q12, Red
D17Z1, 17p11.1-q11.1, Green

The HER2 amplification probe consists of a 347kb probe labelled in red, spanning the HER2 (ERBB2) gene and neighbouring regions, and a green probe for the chromosome 17 centromere.

Probe information

The HER2 (ERBB2) Human Epidermal growth factor Receptor 2 gene, located at chromosome band 17q12, is a member of the Epidermal Growth Factor (EGF) receptor family¹.

It is activated and amplified in 20-30% of breast cancers² and has been correlated with a poor prognosis for the patient³. Treatment of patients carrying the amplification using the monoclonal antibody Herceptin® (Trastuzumab) has been shown to be effective, increasing survival time by specifically targeting cells overexpressing HER2⁴ and removing them from the system.

Similar results have been obtained for a variety of other malignant neoplasms overexpressing HER2 including ovarian⁵, stomach⁶, salivary gland⁷ and small cell lung⁸ cancers.

FFPE tissue preparation and FISH protocol

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Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.