First slide
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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Breast cancer
  • Region 17q12
  • Label    
  • Product No. LPS 001 (10 tests)
    LPS 001-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • HER2 (ERBB2), 17q12, Red
  • D17Z1, 17p11.1-q11.1, Green

The HER2 amplification probe consists of a 347kb probe labelled in red, spanning the HER2 (ERBB2) gene and neighbouring regions, and a green probe for the chromosome 17 centromere.

 

Probe information

The HER2 (ERBB2) Human Epidermal growth factor Receptor 2 gene, located at chromosome band 17q12, is a member of the Epidermal Growth Factor (EGF) receptor family1.

It is activated and amplified in 20-30% of breast cancers2 and has been correlated with a poor prognosis for the patient3. Treatment of patients carrying the amplification using the monoclonal antibody Herceptin® (Trastuzumab) has been shown to be effective, increasing survival time by specifically targeting cells overexpressing HER24 and removing them from the system.

Similar results have been obtained for a variety of other malignant neoplasms overexpressing HER2 including ovarian5, stomach6, salivary gland7 and small cell lung8 cancers.

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References

  1. Coussens L, Yang-Feng TL et al., Science 1985;230(4730):1132-9
  2. Slamon DJ et al., Science 1987;235(4785):177-82
  3. López-Guerrero JA et al., Int J Cancer. 2006 Apr 1;118(7):1743-9
  4. Kauraniemi P et al., Oncogene 2004;23(4):1010-3
  5. Slamon DJ et al., Science 1989;244(4905):707-12
  6. Gravalos C, Jimeno A, Ann Oncol 2008;19(9):1523-9
  7. Vidal L et al., Head Neck 2009;31(8):1006-12
  8. Ugocsai et al., Anticancer Res. 2005;25(4):3061-6

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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