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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Sarcoma
  • Region 16p11.2
  • Label    
  • Product No. LPS 050 (10 tests)
    LPS 050-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • FUS, 16p11.2, Red
  • FUS, 16p11.2, Green

The FUS Breakapart probe consists of one 502kb probe labelled in green, situated distal to the FUS gene and covering markers SHGC-58827 and D16S2660, and three probes (189kb, 160kb and 96kb) labelled in red, situated proximal to the FUS gene and covering markers SHGC-110391 and D16S2933E.

 

Probe information

The FUS (FUS RNA binding protein) gene at 16p11.2 is a member of the FET family of protein-encoding genes, closely-related to the EWSR1 (EWS RNA binding protein 1) gene1.

Recurrent rearrangements involving the FUS gene with a number of different partner genes have been reported in various types of neoplastic disease, notably soft tissue sarcomas and acute myeloid leukaemia. In some tumour types FUS and EWSR1 may replace each other as fusion partners2.

In soft tissue sarcoma, approximately 90% of cases of myxoid liposarcoma are characterised by the presence of a FUS-DDIT3 rearrangement arising from a t(12;16)(q13;p11) translocation3,4; the FUS-CREB3L1 and the FUS-CREB3L2 fusions, resulting from t(11;16) (p11;p11) and t(7;16)( q32-34;p11) translocations respectively are characteristic of low-grade fibromyxoid sarcoma5, whereas the t(12;16)(q13;p11) translocation resulting in a FUS-ATF1 fusion gene is seen in angiomatoid fibrous histiocytoma6.

This breakapart probe has been designed to allow detection of FUS rearrangements regardless of the partner gene involved.

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References

  1. Göransson, M. et al., Oncogene 2009. 28:270–278
  2. Andersson, M.K. et al., BMC cell biology 2008. 9:37
  3. Willeke, M. et al., Clin Cancer Res. 1998. 4:1779–1784
  4. Panagopoulos, I. et al., Cancer Research 1994. 54:6500–6503
  5. Mertens, F. et al., Laboratory investigation 2005. 85:408-15
  6. Tanas, M.R. et al., Modern pathology 2010. 23:93–7

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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