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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Sarcoma
  • Region 22q12.1-q12.2
  • Label    
  • Product Code LPS 006 (10 tests)
    LPS 006-S (5 tests)
  • Regulatory Status In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.



Probe specification

  • EWSR1, 22q12.1-q12.2, Red
  • EWSR1, 22q12.1-q12.2, Green

The EWSR1 Breakapart probe contains a red 392kb probe and a green 631kb probe, which are positioned on each side of the EWSR1 gene.


Probe information

Ewing's sarcoma (EWS) is the second most frequent primary bone cancer in patients under 20 years of age1. It is mainly characterised by a translocation between the two genes - EWSR1 (Ewing's Sarcoma Region 1) and FLI1 (Friend Leukaemia virus Integration 1) - in the t(11;22)(q24;q12) translocation, found in 83% of such tumours2. 10% of the remaining cases have a variant translocation involving EWSR1 and ERG, t(21;22)(q22;q12), and less than 1% carry one of the 7p22 (ETV1), 17q12 (E1AF) or 2q36 (FEV) translocations with EWSR13.

EWSR1 is also involved in translocations in desmoplasmic small round cell tumours, a subset of angiomatoid fibrous histocytomas, and possibly in myxoid liposarcoma4,5.

The EWSR1 breakapart probe can also be used to distinguish rare soft tissue sarcoma, a clear cell sarcoma, from malignant melanoma, a distinction that is difficult to make via either histology or immunohistology. The EWS-ATF1 (Activating Transcription Factor 1) translocation, t(12;22) (q13;q12), has been identified in 70-90% of clear cell sarcoma yet has not been observed in malignant melanoma6. This translocation can be detected using the EWSR1 breakapart probe.

FISH has been shown to be a more sensitive and reliable method than RT-PCR for the diagnosis of EWS in solid tissues7.

What our customers say


  1. Bernstein M et al., Oncologist 2006;11(5):503-19
  2. Turc-Carel C et al., Cancer Genet Cytogenet 1988;32:229-38
  3. Suárez Antelo et al., Arch Bronconeumol. 2010;46(01):44-6
  4. Borden EC et al., Clin Canc Res 2003;9:1941-56
  5. Sandberg AA, Bridge JA, Cancer Genet Cytogenet 2000;123(1):1-26
  6. Patel RM et al., Modern Path 2005;18:1585-90
  7. Bridge RS et al., Modern Path 2006;19:1-8

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

1. Heat Pretreatment Dark Blue
  • Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
  • Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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