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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lung cancer
  • Region 2p21
  • Label    
  • Product No. LPS 020 (10 tests)
    LPS 020-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • EML4, 2p21, Red
  • EML4, 2p21, Green

The EML4 Breakapart probe consists of a green 406kb probe and a red 353kb probe, which are positioned on each side of the EML4 gene.

 

Probe information

The protein encoded by the echinoderm microtubule associated protein-like 4 (EML4) gene is involved in microtubule formation and stabilisation1.

A novel gene fusion of EML4 and Anaplastic Lymphoma Kinase (ALK) was recently identified in Non-Small Cell Lung Cancer (NSCLC).

The EML4-ALK fusion was shown to result from an inversion within chromosome 2p, inv(2)(p21p23), and was detected in approximately 7% of NSCLC cases2,3.

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References

  1. Xuchao Zhang et al., Mol Cancer Res 2010;9:188
  2. Lin et al., Mol Cancer Res 2009;7(9):1466-1476
  3. Martelli et al., AJP 2009;174(2):661-670

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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