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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest Lymphoma
  • Region 3q27.3
  • Label    
  • Product No. LPH 035 (10 tests)
    LPH 035-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • BCL6, 3q27.3, Red
  • BCL6, 3q27.3, Green

The BCL6 product consists of a 165kb probe, labelled in red, centromeric to the BCL6 gene and a green probe, covering a 170kb region telomeric to the BCL6 gene.

 

Probe information

Chromosomal rearrangements involving the BCL6 (B-cell CLL/lymphoma 6) gene at 3q27 are recognised recurrent abnormalities commonly seen in patients with B-cell malignancy1.

BCL6 rearrangements are the most common chromosomal abnormalities seen in diffuse large B-cell lymphoma (DLBCL), occurring in up to 35% of cases2. They are also seen frequently in follicular lymphoma, where they occur in up to 15% of cases3. BCL6 is expressed in normal germinal centre B-cells and follicle helper T-cells. BCL6 translocations alter expression by promoter substitution and cause deregulated expression of normal BCL6 protein1,4.

Approximately 50% of BCL6 translocations will involve one of the three immunoglobulin loci (IGH, IGL or IGK); the remainder of translocations involve one of more than twenty different non-immunoglobulin genes5. Additionally, gains and amplifications of the BCL2 gene have also been reported in cases of B-cell lymphoma6.

The presence of concurrent BCL6 rearrangements with MYC and/or BCL2 rearrangements in patients with ‘dual-hit’ lymphoma has been shown to be associated with aggressive disease7.

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References

  1. Wagner SD et al., Br J Haematol 2011;152(1):3-12
  2. Lossos I et al., Leukemia 2003;17(7):1390-7
  3. Akasaka T et al., Blood. 2003;102(4):1443-8
  4. Ye BH, et al. EMBO J 1995;14(24):6209-17
  5. Ohno H, J Clin Exp Hematop 2006;46(2):43-53
  6. Karube K, et al., Mod Pathol 2008;21(8):973-8
  7. Aukema SM, et al., Blood. 2011;24;117(8):2319-31

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.

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