First slide
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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest CLL
  • Region 12p11.1-q11.1
  • Label  
  • Product No. LPH 069 (10 tests)
    LPH 069-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • D12Z3, 12p11.1-q11.1, Red

The Alpha Satellite 12 Plus Probe is a repeat sequence probe, labelled in red, which recognises the centromeric repeat sequence D12Z3.

 

Probe information

Trisomy 12 is a recurrent abnormality in CLL, seen in 20% of cases1 that often appears as the unique cytogenetic aberration (40-60% of cases with trisomy 12)2. Patients with trisomy 12 are classified as low-risk in the absence of any other genetic lesions3.

This product has been optimised for overnight hybridisation and is also available as part of the CLL Plus Screening Panel (LPH 087).

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References

  1. Swerdlow et al., (eds,) WHO Classification of Tumours of Haematopoietic and Lymphoid Tissue, Lyon, France, 4th edition, IARC,2017
  2. Puiggros et al., Biomed Res Int 2014;1-13
  3. Rossi et al., Blood 2013;121(8):1403-1412

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.

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