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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lung cancer
  • Region 2p23.2-p23.1
  • Label    
  • Product No. LPS 019 (10 tests)
    LPS 019-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • ALK, 2p23.2-p23.1, Red
  • ALK, 2p23.2-p23.1, Green

The ALK Breakapart probe consists of a green 420kb probe, which spans the majority of the ALK gene and a red 486kb probe, which is telomeric to the ALK gene.

 

Probe information

Transforming rearrangements of the Anaplastic Lymphoma Receptor Tyrosine Kinase (ALK) gene at 2p23 have been recognised in a subset of human haematological and solid malignancies1.

ALK fuses with Nucleophosmin (NPM1) in anaplastic lymphoma, resulting in constitutive kinase activity, which inhibits apoptosis and promotes cellular proliferation2. The rearrangement of ALK has now also been implicated in the development of Non-Small Cell Lung Cancer (NSCLC) as a result of an inversion involving chromosome 2, inv(2)(p21p23), causing ALK to fuse with the EML4 gene2. The ALK gene is frequently involved in translocations of chromosome 2 that lead to gene fusions in a variety of different malignancies, including neuroblastomas and myofibroblastic tumours3.

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References

  1. Marileila Varella-Garcia et al., Association of molecular pathology: Solid Tumour Review. 2010
  2. Takeuchi K et al., Clin Cancer Res. 2008;14(20):6618-6624
  3. Lin et al., Mol Cancer Res. 2009;7(9):1466-1476

FFPE tissue preparation and FISH protocol

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Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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