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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest CLL
  • Region 13q14.2-q14.3
    13q34
  • Label    
  • Product No. LPH 006 (10 tests)
    LPH 006-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • 13q14.2-q14.3, Red
  • 13qter, 13q34, Green

The 13q14.2-q14.3 probe, labelled in red, covers the D13S319 and D13S25 markers. The 13qter subtelomere specific probe (clone 163C9), labelled in green, allows identification of chromosome 13 and acts as a control probe.

 

Probe information

Chromosome 13q abnormalities occur in 16-40% of multiple myeloma cases and are associated with poor prognosis1,2. A case study has shown that in 90% of patients, the 13q14 region was affected and 68% also showed involvement of the 13q21 region – the critical region in all but 8 patients was narrowed down to 13q143. Deletions affecting 13q14 are also the most frequent structural genetic abnormalities in B-cell Chronic Lymphocytic Leukaemia (B-CLL)4. This region is found to be heterozygously deleted in 30-60% and homozygously deleted in 10-20% of CLL patients5. Recently though, the survival rate has been shown to be similar for the two groups6. Two non-coding RNA genes, DLEU1 and DLEU2, and the genetic marker D13S319, span the pathogenic critical region of 13q147. DLEU1 is considered to be the most likely CLL-associated candidate tumour suppressor gene within the 13q14 region8. Subsequently, D13S319, located between the RB1 gene and D13S25 and within the DLEU1 locus, was found to be deleted in 44% of CLL cases9. It has also been postulated that a gene telomeric to the D13S319 region, encompassing D13S25, may be important in cases with hemizygous deletions and that this gene is a putative tumour suppressor gene10.

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References

  1. Bullrich F et al., Cancer Res 2001;61:6640-8
  2. Zojer et al., Blood 2000;95(6):1925-1930
  3. Shaughnessy J et al., Blood 2000;96:1505-11
  4. Juliusson G et al., N Eng J Med 1990;323:720-4
  5. Hammarsund M et al., FEBS Letters 2004;556:75-80
  6. Van Dyke DL et al., Br J Haematology 2009;148:544-50
  7. Liu Y et al., Oncogene 1997;15:2463-73
  8. Wolf S et al., Hum Mol Genet 2001;10:1275-85
  9. Liu Y et al., Blood 1995;86:1911-5
  10. Bullrich F et al., Blood 1996;88(8):3109-15

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.
Haematology FISH protocol Video Image
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Haematology FISH protocol

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