SureSeq myPanel™ NGS Custom Prostate Cancer Panel

SureSeq myPanel offers:

• Hybridisation-based enrichment delivering unparalleled coverage uniformity — detect low frequency prostate cancer variants consistently with confidence and minimise the requirement for supplementary fill-in with Sanger sequencing
• Pre-optimised panels that meet your technical requirements and work with your samples — no more lengthy in-house optimisation, decreasing assay development time
• Bespoke panel content — sequence only what’s relevant for your cancer research, increase throughput and save on sequencing reagents
• Panel content designed with experts and from current literature to target all relevant regions including intronic and splice sites — get the most comprehensive insight into disease-driving mutations

Getting started with SureSeq myPanel NGS Custom Prostate Cancer Panel could not be simpler...

Simply mix and match the genes or individual exons you require and get the most out of your sequencing runs.

Ordering Information

SureSeq: For research use only; not for use in diagnostic procedures.

Prostate cancer is now the second leading cause of cancer in men, with recent genome-wide studies helping to clarify the genetic basis of this common but complex disease¹. Many of these studies have reinforced the importance of homologous end repair genes including: ATM, BRCA1, BRCA2 and PALB2, in the mechanism of prostate cancer development. Mutations in these genes result in cells having to repair lesions through other non-conservative mutagenic mechanisms.

Choose your ideal prostate cancer NGS panel from our range of fully optimised NGS panel content. Simply mix and match the genes or individual exons you require and get the most out of your sequencing runs. Use in conjunction with the SureSeq FFPE DNA Repair Mix* for improved NGS library yields, %OTR (on target rate) and mean target coverage from challenging FFPE derived samples.

Superior Coverage Uniformity

A number of genetic factors have been found that increase prostate cancer risk, including heritable mutations in the genes BRCA1 and BRCA2. BRCA1 is a key player in cellular control systems, having been linked to DNA damage response and repair, transcriptional regulation and chromatin modelling², while BRCA2 function is linked to DNA recombination and repair processes, being of particular importance in the regulation of RAD51 activity.

Figure 1a, illustrates the superior uniformity of coverage of key exons of BRCA1, and Figure 1b, BRCA2 from an FFPE sample.

Figure 1a

Figure 1b

Figure 1a. BRCA1 exon 9 and 10 coverage. Figure 1b. BRCA2 exon 11 coverage. Depth of coverage per base (grey). Targeted region (green). Gene coding region as defined by RefSeq (blue). GC percentage (red).

PALB2 is a BRCA2 binding protein and the BRCA2-PALB2 interaction is essential for BRCA2-mediated DNA repair. Recently it has been shown that correct PALB2 function is necessary for the homologous recombination repair via interaction with BRCA1, revealing that PALB2 is actually a linker between BRCA1 and BRCA2³. The ATM gene, located on chromosome 11q 22–23, includes 66 exons with a 9168 base pair coding sequence, and encodes a PI3K-related protein kinase (PIKK) that helps maintain genomic integrity. The PI3K-AKT-mTOR oncogenic pathway is frequently enhanced in prostate cancer playing a vital role in development and maintenance⁴.

Figures 2 and 3 illustrate the excellent uniformity of coverage of key exons of PALB2 and ATM, respectively.

Figure 2a

Figure 2b

Figure 3a

Figure 3b

Figures 3a and 3b. Illustration of the excellent uniformity of coverage of PALB2 exons 5 (2a) and 13 (2b) and ATM exons 45 (3a) and 62 (3b). Depth of coverage per base (grey). Targeted region (green). Gene coding region as defined by RefSeq (blue). GC percentage (red).

Getting started with your next SureSeq myPanel Custom Cancer Panel could not be simpler. 

Select from any of the following myPanel Prostate Cancer whole gene or exonic content below:

Talk to us about your custom breast cancer NGS requirements and let our expertise work in helping you to advance your cancer research…

Ordering Information

*The SureSeq FFPE DNA Repair Mix can only be purchased in conjunction with SureSeq NGS panels, not as a standalone product.

References

1. Thoma, C, (2015) The complex relationships of malignant cells in lethal metastatic castration-resistant disease, Nature Reviews Urology 12, 237
2. Castro, E. et al, (2012) The role of BRCA1 and BRCA2 in prostate cancer. Asian Journal of Andrology, 14 (3):409-414.
3. Pakkanen, S. et al, (2009) PALB2 variants in hereditary and unselected Finnish Prostate cancer cases. Journal of Negative Results in BioMedicine, 8 (1).
4. Angèle, S. et al, (2004). ATM polymorphisms as risk factors for prostate cancer development. British Journal of Cancer, 91(4): 783–787.

SureSeq: For research use only; not for use in diagnostic procedures.