SureSeq™ NGS Library Preparation Kit

Applications:
,
Product types:
,

Streamlined library preparation for industry-leading next generation sequencing (NGS) results.

Find out more

For more information about any OGT product or service, please contact us.

Overview

The SureSeq NGS Library Preparation Kit generates NGS libraries suitable for the capture of targeted genomic regions using hybridisation. Optimisation of enzymes and buffers enables the number of steps to be reduced, while still delivering libraries of the highest quality.

The SureSeq Library Preparation Kit delivers:

  • Greater trust in your data — High performance with low duplication rates, high sequence quality and high percentage of on-target bases
  • Faster process — Streamlined protocol reduces manual handling steps to save time, increasing reliability as well as throughput
  • Reliable results — NGS targeted panel, NGS library preparation and powerful NGS analysis software are fully optimised for perfect results every time

Ordering Information

Product Contents Cat. No. Price
SureSeq NGS Library Preparation Kit (16)  Bundle of 1 x library preparation kit (16) containing adaptors, PCR primers and enzymes sufficient for 16 samples and 1 x SureSeq NGS Index Kit – Collection A 500070 Get a quote
SureSeq NGS Library Preparation Kit (48)  Bundle of 3 x library preparation kit (16), containing adaptors, PCR primers and enzymes sufficient for 48 samples and 1 x SureSeq NGS Index Kit – Collection B 500073 Get a quote
SureSeq NGS Index Kit - Collection A (16) 16 different indexes, each sufficient for 4 samples [included with SureSeq NGS Library Preparation Kit (16)] 500071 Get a quote
SureSeq NGS Index Kit - Collection B (48) 48 different indexes, each sufficient for 4 samples [included with SureSeq NGS Library Preparation Kit (48)] 500072 Get a quote


SureSeq: For research use only; not for use in diagnostic procedures.

Details

Greater trust in your data

NGS data is increasingly being relied upon as a front line technology for the generation of data for scientific and medical research. A combination of quality metrics are used to give confidence that variants called are real and the number of false positive calls are low. The SureSeq NGS Library Preparation Kit delivers high performance in the quality metrics that really matter, giving more reliable, more trustworthy data.

Low levels of duplication

High duplication rates reduce the complexity of your NGS library and can lead to poor coverage of target genomic regions, even though average coverage rates appear high. The SureSeq NGS Library Preparation Kit gives exceptionally low levels of duplication (Figure 1). This ensures more accurate calling, more even coverage and higher levels of confidence in the data produced.

LPK Figure 1

Figure 1: The SureSeq NGS Library Preparation Kit delivers low levels of sequence duplication. The duplication rates are shown for 8 samples sequenced using exome capture (Agilent SureSelect Human All Exon V5) with the SureSeq NGS Library Preparation Kit and a library preparation kit from an alternative supplier.

High-quality sequence data

For complete confidence in your data, the sequence quality scores should be high and consistent across the length of each read. The SureSeq NGS Library Preparation Kit delivers exceptional sequence quality scores even 100 bases into the read (Figure 2). This reduces the requirement for post-acquisition bioinformatics, such as read size reduction, to remove poor quality sequence, giving you complete confidence in your data.

LPK Figure 2Figure 2: The SureSeq NGS Library Preparation Kit delivers high sequence quality. Data shown
was generated using human genomic DNA, the OGT SureSeq Myeloid Panel and SureSeq NGS
Library Preparation Kit

High levels of on-target bases

In order to maximize the amount of useful information that can be obtained for a given sequencing lane, high levels of on-target bases are required. The benefits of superior levels of specific sequence capture include, increased sequence complexity and greater depth of coverage of the target regions. The SureSeq NGS Library Preparation Kit delivers a high percentage of on-target bases compared to other library preparation kits (Figure 3).

LPK Figure 3Figure 3: High percentage of on-target bases. The percentage of on-target bases are shown for exome capture (Agilent SureSelect Human All Exon V5) using the SureSeq NGS Library Preparation Kit and a library preparation kit from an alternative supplier.

Faster process with streamlined protocol

By extensive testing of enzymes and buffer optimisation, it has been possible to streamline the standard library preparation protocol to reduce the number of hands-on steps and the overall processing time (Figure 4).

  • End repair and ligation are combined into a single step
  • Purification steps, where material is lost, are reduced
  • Fewer steps reduce handling errors for increased reliability
  • Reduced technician cost and increased sample throughput

LPK Figure 4Figure 4: A streamlined protocol, including enrichment by hybridisation. The complete procedure can be completed in 1.5 days with minimal handling time.

Reliable results with full optimisation with SureSeq targeted panels and analysis software

The SureSeq NGS Library Preparation Kit works hand-in-hand with our targeted enrichment panels, which have been developed with world-leaders in their respective fields. This ensures you get the most sensitive and reproducible variant detection and industry-leading coverage uniformity.

SureSeq Interpret Software — OGT’s powerful, standalone data analysis and reporting package — is provided free with all our SureSeq targeted panels to convert FASTQ files into an intuitive interactive report. Variants are fully annotated with links to various databases (e.g. dbSNP, COSMIC, Genecards and OMIM) providing results in context.

Ordering Information

Product Contents Cat. No. Price
SureSeq NGS Library Preparation Kit (16) Bundle of 1 x library preparation kit (16) containing adaptors, PCR primers and enzymes sufficient for 16 samples and 1 x SureSeq NGS Index Kit – Collection A 500070 Get a quote
SureSeq NGS Library Preparation Kit (48)  Bundle of 3 x library preparation kit (16), containing adaptors, PCR primers and enzymes sufficient for 48 samples and 1 x SureSeq NGS Index Kit – Collection B 500073 Get a quote
SureSeq NGS Index Kit - Collection A (16) 16 different indexes, each sufficient for 4 samples [included with SureSeq NGS Library Preparation Kit (16)] 500071 Get a quote
SureSeq NGS Index Kit - Collection B (48) 48 different indexes, each sufficient for 4 samples [included with SureSeq NGS Library Preparation Kit (48)] 500072 Get a quote
SureSeq Ovarian Cancer Panel 16 reactions Enrichment baits sufficient for 16 samples; SureSeq Interpret Software 600073 Get a quote
SureSeq Ovarian Cancer Panel 96 reactions Enrichment baits sufficient for 16 samples; SureSeq Interpret Software 600074 Get a quote
SureSeq Myeloid Panel 16 reactions Enrichment baits sufficient for 16 samples; SureSeq Interpret Software 600075 Get a quote
SureSeq Myeloid Panel 96 reactions Enrichment baits sufficient for 16 samples; SureSeq Interpret Software 600076 Get a quote


SureSeq: For research use only; not for use in diagnostic procedures

The SureSeq NGS Library Preparation Kit was jointly developed between Oxford Gene Technology and Bioline Reagents Limited.