SureSeq™ NGS Library Preparation Kit
Streamlined library preparation for industry-leading next generation sequencing (NGS) results.
The SureSeq NGS Library Preparation Kit generates NGS libraries suitable for the capture of targeted genomic regions using hybridisation. Optimisation of enzymes and buffers enables the number of steps to be reduced, while still delivering libraries of the highest quality.
The SureSeq Library Preparation Kit delivers:
- Greater trust in your data — High performance with low duplication rates, high sequence quality and high percentage of on-target bases
- Faster process — Streamlined protocol reduces manual handling steps to save time, increasing reliability as well as throughput
- Reliable results — NGS targeted panel, NGS library preparation and powerful NGS analysis software are fully optimised for perfect results every time
Greater trust in your data
NGS data is increasingly being relied upon as a front line technology for the generation of data for scientific and medical research. A combination of quality metrics are used to give confidence that variants called are real and the number of false positive calls are low. The SureSeq NGS Library Preparation Kit delivers high performance in the quality metrics that really matter, giving more reliable, more trustworthy data.
Low levels of duplication
High duplication rates reduce the complexity of your NGS library and can lead to poor coverage of target genomic regions, even though average coverage rates appear high. The SureSeq NGS Library Preparation Kit gives exceptionally low levels of duplication (Figure 1). This ensures more accurate calling, more even coverage and higher levels of confidence in the data produced.
Figure 1: The SureSeq NGS Library Preparation Kit delivers low levels of sequence duplication. The duplication rates are shown for 8 samples sequenced using exome capture (Agilent SureSelect Human All Exon V5) with the SureSeq NGS Library Preparation Kit and a library preparation kit from an alternative supplier.
High-quality sequence data
For complete confidence in your data, the sequence quality scores should be high and consistent across the length of each read. The SureSeq NGS Library Preparation Kit delivers exceptional sequence quality scores even 100 bases into the read (Figure 2). This reduces the requirement for post-acquisition bioinformatics, such as read size reduction, to remove poor quality sequence, giving you complete confidence in your data.
High levels of on-target bases
In order to maximize the amount of useful information that can be obtained for a given sequencing lane, high levels of on-target bases are required. The benefits of superior levels of specific sequence capture include, increased sequence complexity and greater depth of coverage of the target regions. The SureSeq NGS Library Preparation Kit delivers a high percentage of on-target bases compared to other library preparation kits (Figure 3).
Figure 3: High percentage of on-target bases. The percentage of on-target bases are shown for exome capture (Agilent SureSelect Human All Exon V5) using the SureSeq NGS Library Preparation Kit and a library preparation kit from an alternative supplier.
Faster process with streamlined protocol
By extensive testing of enzymes and buffer optimisation, it has been possible to streamline the standard library preparation protocol to reduce the number of hands-on steps and the overall processing time (Figure 4).
- End repair and ligation are combined into a single step
- Purification steps, where material is lost, are reduced
- Fewer steps reduce handling errors for increased reliability
- Reduced technician cost and increased sample throughput
Reliable results with full optimisation with SureSeq targeted panels and analysis software
The SureSeq NGS Library Preparation Kit works hand-in-hand with our targeted enrichment panels, which have been developed with world-leaders in their respective fields. This ensures you get the most sensitive and reproducible variant detection and industry-leading coverage uniformity.
SureSeq Interpret Software — OGT’s powerful, standalone data analysis and reporting package — is provided free with all our SureSeq targeted panels to convert FASTQ files into an intuitive interactive report. Variants are fully annotated with links to various databases (e.g. dbSNP, COSMIC, Genecards and OMIM) providing results in context.
|SureSeq NGS Library Preparation Kit (16)||Bundle of 1 x library preparation kit (16) containing adaptors, PCR primers and enzymes sufficient for 16 samples and 1 x SureSeq NGS Index Kit – Collection A||500070||Get a quote|
|SureSeq NGS Library Preparation Kit (48)||Bundle of 3 x library preparation kit (16), containing adaptors, PCR primers and enzymes sufficient for 48 samples and 1 x SureSeq NGS Index Kit – Collection B||500073||Get a quote|
|SureSeq NGS Index Kit - Collection A (16)||16 different indexes, each sufficient for 4 samples [included with SureSeq NGS Library Preparation Kit (16)]||500071||Get a quote|
|SureSeq NGS Index Kit - Collection B (48)||48 different indexes, each sufficient for 4 samples [included with SureSeq NGS Library Preparation Kit (48)]||500072||Get a quote|
|SureSeq Ovarian Cancer Panel 16 reactions||Enrichment baits sufficient for 16 samples; SureSeq Interpret Software||600073||Get a quote|
|SureSeq Ovarian Cancer Panel 96 reactions||Enrichment baits sufficient for 16 samples; SureSeq Interpret Software||600074||Get a quote|
|SureSeq Myeloid Panel 16 reactions||Enrichment baits sufficient for 16 samples; SureSeq Interpret Software||600075||Get a quote|
|SureSeq Myeloid Panel 96 reactions||Enrichment baits sufficient for 16 samples; SureSeq Interpret Software||600076||Get a quote|
The SureSeq NGS Library Preparation Kit was jointly developed between Oxford Gene Technology and Bioline Reagents Limited.
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SureSeq™ FFPE DNA Repair Mix
SureSeq™ NGS Library Preparation Kit
SureSeq™ Ovarian Cancer Panel
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DNA fragmentation is a crucial first step in the preparation of libraries for NGS. In this application note, Oxford Gene Technology has evaluated an alternative method of fragmentation using the NEBNext® dsDNA Fragmentase®.
Selecting the best NGS enrichment assay for your needs
With NGS now in routine use for a broad range of research and clinical applications, this application note details the value of making the correct choice for the initial sequence enrichment step.
SureSeq myPanel™ NGS Custom Cancer Panels Full Gene List
We now have 120 genes available for our SureSeq myPanel NGS Custom Cancer Panels. View and download a complete list of available cancer gene content.
Optimised, 1-day hybridisation-based NGS protocol yields 1% variant detection in MPN samples, as quickly and cost-effectively as multiplex PCR
Presented at AMP 2016, this poster outlines how the SureSeq™ Core MPN Panel can accurately detect alleles down to 1% variant allele fraction (VAF) in JAK2 (V617F) at a read depth of >1000x, facilitating reliable detection.
The accurate detection by next-generation sequencing (NGS) of difficult to sequence genes (CALR, CEBPA, FLT3) associated with myeloid disorders using a hybridisation-based enrichment approach
Presented at CGC 2017, this poster highlights the excellent uniformity of coverage obtained from the hybridisation-based enrichment using the SureSeq myPanel NGS Custom AML Panel.
The analysis of myeloproliferative neoplasm samples using a rapid (30 minute) hybridisation-based enrichment protocol for next-generation sequencing (NGS)
Presented at the CGC 2017 annual summer meeting in Denver, USA, this poster illustrates the excellent quality data generated by the OGT 1-day hybridisation-based SureSeq LPK protocol in combination with the SureSeq Core MPN Panel.
The application of a hybridisation-based next-generation sequencing (NGS) enrichment panel for the analysis of key genes involved in ovarian and breast tumours using DNA from FFPE samples
The application of a hybridisation-based NGS enrichment panel for the analysis of somatic variants in tumour samples and reference standards
Presented at AGT 2017, this poster outlines the application of a hybridisation-based NGS enrichment panel for the analysis of solid tumour somatic variants, demonstrating 100% concordance in variant detection in both genomic and formalin-compromised DNA.
The application of a one-day hybridisation-based enrichment protocol for NGS incorporating a rapid (30 minute) hybridisation step
Presented at AGT 2017, this poster outlines how OGT has optimised a one-day hybridisation-based enrichment protocol for NGS incorporating a rapid 30 hybridisation step.
The use of a hybridisation-based NGS enrichment panel for the confident identification of a broad range of low frequency variants from as little as 50ng of challenging clinical research FFPE samples
Presented at AMP 2016, this poster outlines how the SureSeq FFPE DNA Repair Mix significantly improves NGS library yields, with an increase of mean target coverage (increased by >2.2 fold), resulting in more meaningful data.
The role of NGS in stratified cancer medicine
In this white paper, two Clinical Scientists, Dr Matthew Smith and Dr George Burghel, share their views on the use of NGS in cancer genomics and its integration into the laboratory.
Understanding myeloid disorders with next-generation sequencing
How OGT’s SureSeq™ Myeloid Panel helps researchers identify and decipher the complex genetic origins of myeloproliferative disorders