SureSeq myPanel™ NGS custom FH panel

The SureSeq myPanel NGS custom FH panel offers:

• Hybridisation-based enrichment delivering unparalleled coverage, completeness and uniformity — detect variants with confidence and minimise the requirement for supplementary fill-in with Sanger sequencing
• Detection of copy number variation (CNV) as well as single nucleotide variants (SNV)  with a single assay
• Pre-optimised panels that meet your technical requirements — no more laborious in-house optimisation, decreasing assay development time
• Bespoke panel content — sequence only what’s relevant for your research, increase throughput and save on sequencing reagents
• Custom CytoSure microarrays — available for confirmation of CNV detection

Getting started with your next SureSeq myPanel NGS Custom FH Panel could not be simpler...

Simply mix and match the gene and hotspot baits that you require for your research to enable analysis of both SNV and CNV from a single assay.

Ordering Information

SureSeq: For research use only; not for use in diagnostic procedures.

Simply mix and match the gene and hotspot baits that you require for your research to enable analysis of both SNV and CNV from a single assay.

Select from:

All exons*

Hotspots*

*Some genes/hotspots may not be available in your region – contact us for more details

CNV and SNV from a single assay

The hybridisation methodology, combined with our bait design expertise, allows generation of panels with outstanding completeness and coverage uniformity. Together, this allows the areas of CNV to be easily identified within each sample using our proprietary algorithm — delivering an increased understanding of the sample without an increase in cost or time.

A customisable CytoSure microarray for confirmation of CNV using array comparative genomic hybridisation (aCGH) is also available. On an 8x15k array format : ABCG8, APOA5, APOB, APOC2, APOC3, APOE, LDLR, LDLRAP1, LIPA, LPL, PCSK9, and STAP1 were covered by probes giving exon-level resolution, in addition to probes covering the genomic backbone.

Figure 1 (below): CNV in LDLR gene shown using IGV from the Broad Institute (A).

(A) Red bars indicate areas of CNV (data from aCGH), purple bars represent deleted exons (data from NGS): 5 samples are shown, each with at least one area of CNV. There is complete concordance between the aCGH and NGS data. Note the evenness of the NGS coverage (even peak height) across each exon, allowing the areas of CNV to be easily identified. The data from the custom CytoSure aCGH array, confirms the deletions in LDLR.

(B) A 2 exon deletion corresponding to sample 3 in A.

(C) A deletion of 2 exons and 4 exons, corresponding to sample 5 in A.

Getting started with SureSeq myPanel NGS Custom FH Panel could not be simpler...

Talk to us today about your FH custom my panel

Simply mix and match the gene and hotspot baits that you require for your research to enable analysis of both SNV and CNV from a single assay.

An FH microarray for confirmation of CNV is also available. Please enquire for further details.

SureSeq: For research use only; not for use in diagnostic procedures.