Array Banner

Ruth Burton and Douglas Hurd



Array comparative genomic hybridization (aCGH) is a well-established technique used to detect chromosomal abnormalities, particularly in conditions such as development delay and intellectual disabilities. To identify potentially disease causative genomic regions, DNA from the test sample is labelled with one fluorescent dye, usually Cyanine 5 and a second reference sample is labelled with a different fluorescent dye, typically Cyanine 3. The two labelled samples are then competitively hybridized to a microarray. Differences in copy number between the test and reference sample are calculated by measuring the signal intensities of the two dyes and converting these measurements to a ratio. Efficient labeling of both test and reference samples is a critical step in the microarray process and poor labeling can result in inaccurate and potentially misleading data.

“Our lab has tried a variety of different labelling methods; and found OGT’s CytoSure Genomic DNA Labelling Kit to give consistently low DLRS values and excellent signal-to-noise ratios”This application note provides a technical evaluation of CytoSure® Genomic DNA Labelling Kits compared with another leading DNA labeling kit. CytoSure Genomic DNA Labelling Kits are shown to improve key microarray quality control (QC) metrics and consistently generate data with improved signal-to-noise ratios and low derivative log ratio spreads (DLRS).

Register with us to read the full article

Once you have registered with us for free you will be able to read all our supportive literature, video tutorials and webinars.

  • Share
You might also be interested in
CTA Icon

Stay up-to-date with the latest news from OGT, including new products, support resources, and our DNA Dispatch newsletter