Ruth Burton and Douglas Hurd
Array comparative genomic hybridization (aCGH) is a well-established technique used to detect chromosomal abnormalities, particularly in conditions such as development delay and intellectual disabilities. To identify potentially disease causative genomic regions, DNA from the test sample is labelled with one fluorescent dye, usually Cyanine 5 and a second reference sample is labelled with a different fluorescent dye, typically Cyanine 3. The two labelled samples are then competitively hybridized to a microarray. Differences in copy number between the test and reference sample are calculated by measuring the signal intensities of the two dyes and converting these measurements to a ratio. Efficient labeling of both test and reference samples is a critical step in the microarray process and poor labeling can result in inaccurate and potentially misleading data.
This application note provides a technical evaluation of CytoSure® Genomic DNA Labelling Kits compared with another leading DNA labeling kit. CytoSure Genomic DNA Labelling Kits are shown to improve key microarray quality control (QC) metrics and consistently generate data with improved signal-to-noise ratios and low derivative log ratio spreads (DLRS).
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