Quantitate your gDNA using a Nanodrop spectrophotometer or a fluorometer e.g. Qubit.
Pete Gray
Array and NGS Field Application Specialist, Europe
Tips include how to dispense hybridization mix to the center of the gasket chamber, why you should monitor the temperature of the hybridization oven, and why it is essential to dislodge stuck bubbles in the hybridization chamber.
Identify and resolve common problems that occur during the array protocol, including bubble scarring/scotching, poor signal intensity and low Cy5 signal towards the edges of a feature.
Learn about the workflow and basic feature of our software with our quick overview video to CytoSure Interpret, a powerful and easy-to-use package for the analysis of aCGH data.