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Product summary

  • Technology NGS
  • Product No. Various
  • Intended use For research use only; not for use in diagnostic procedures.

Overview

Introduction

The SureSeq™ NGS Library Preparation Kit generates NGS libraries suitable for the capture of targeted genomic regions using hybridization. With a streamlined workflow, significantly reduced hands-on steps and hybridization times as low as 30 minutes, SureSeq offers all of the benefits of hybridization in as little as a 1-day workflow. For increased convenience and flexibility, the SureSeq workflow can be performed with an enzymatic or Covaris® DNA fragmentation and Speedvac® or bead concentration steps, whilst still delivering libraries of the highest quality. The inclusion of the SureSeq Hyb & Wash buffer, optimized for use with SureSeq NGS panels, simplifies this key step while offering excellent coverage uniformity and reproducibility. Additionally, a new automation workflow enables standardization of OGT’s library preparation, hybridization and washing steps for up to 96 samples in a single batch, improving laboratory productivity.

Features
  • Greater trust in your data - high performance with low duplication rates, high sequence quality and high percentage of on-target bases
  • Simpler hybridization - all components of the SureSeq Hyb & Wash buffer are ready-to-use with no requirement for multiple wash buffers
  • Rapid process - streamlined protocol, minimal manual handling, automation and a rapid hybridization step offers increased reliability as well as throughput
  • Reliable results - NGS targeted panel, complete NGS library preparation solution and powerful NGS analysis software are fully optimized for excellent results

Capabilities

Greater trust in your data

NGS data is increasingly being relied upon as a front-line technology for the generation of data for scientific and medical research. A combination of quality metrics are used to give confidence that variants called are real and the number of false positive calls are low. The SureSeq NGS Library Preparation Kit delivers high performance in the quality metrics that really matter, giving more reliable, more trustworthy data.

Low levels of duplication

High duplication rates reduce the complexity of your NGS library and can lead to poor coverage of targeted genomic regions even though average coverage rates appear high.

The SureSeq NGS Library Preparation Kit gives exceptionally low levels of duplication (Figure 1). This ensures more accurate calling, more even coverage and higher levels of confidence in the data produced.

High quality sequence data

For complete confidence in your data, the sequence quality scores should be high and consistent across the length of each read. The SureSeq NGS Library Preparation Kit delivers exceptional sequence quality scores even 150 bases into the read (Figure 2). This reduces the requirement for post-acquisition bioinformatics, such as read size reduction, to remove poor quality sequence.

High levels of on-target bases

In order to maximize the amount of useful information that can be obtained for a given sequencing lane, high coverage depth and low coefficients of variation are desirable. The benefits of superior levels of specific sequence capture include increased sequence complexity and greater depth of coverage of the target regions. The SureSeq NGS Library Preparation Kit delivers excellent reproducibility (Figure 3).

Simpler hybridization with the SureSeq NGS Hyb & Wash Kit

The SureSeq NGS Hyb & Wash kit is an easy to use, robust and high performing hybridization solution, containing all the components required to perform the hybridization and wash steps in SureSeq sequence capture protocols. All components in the kit are ready-to-use, eliminating the requirement to dilute buffers and the possibility of cross-contamination during buffer preparation. There is no need to use multiple wash buffers, as in other manufacturer’s kits, the simplified protocol utilizes just a single wash buffer. With hybridization times as little as 30 minutes, it offers excellent quality sequencing data, when used in conjunction with the SureSeq NGS Library Preparation Kit and SureSeq NGS panels (Figure 4).

Rapid process with streamlined protocol

By extensive testing of enzymes and buffer optimization, it has been possible to streamline the standard library preparation protocol to minimize the number of hands-on steps and the overall processing time (Figure 5). For added convenience, OGT now offers Dynabeads™ M270 Streptavidin and AMPure® XP beads for use with SureSeq NGS panels. While the addition of a workflow for automation on the Agilent Bravo A® Automated Liquid Handling Platform offers greater flexibility and productivity for your laboratory.

  • End repair and ligation are combined into a single step
  • Purification steps, where material is lost, are reduced
  • Fewer steps reduce handling errors for increased reliability
  • Reduced technician time and cost and increased sample throughput
  • Workflow amenable to automation
Reliable results with SureSeq targeted NGS panels and analysis software

The SureSeq NGS Library Preparation Kit and Hyb & Wash Kit work hand-in-hand with SureSeq targeted enrichment panels, ensuring you get the most sensitive and reproducible variant detection and industry-leading coverage uniformity (Figure 6).

Interpret is OGT’s powerful and easy-to-use next-generation sequencing analysis solution, facilitating analysis and visualization of a wide range of variants and structural aberrations. Coupled with a comprehensive and powerful filtering framework, the software delivers accurate calling of SNVs and indels, as well as structural aberrations, including ITDs, PTDs, CNVs, LOH and translocations. Interpret is designed to work seamlessly with all SureSeq NGS panels and offers flexible accessibility for data analysis; whether through a stand-alone computer*, laboratory server or another web-enabled device. With a wide range of customization options and links to various mutation databases, Interpret provides effortless translation of all your NGS data into meaningful results.

SureSeq and CytoSure NGS workflow

Library preparation Image

Library preparation

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Sequencing Image

Sequencing

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  • Illumina sequencers

What our customers say

Notes

  1. *Computer specification: RAM: min 16GB, suggested 24GB; HDD: min 500GB, suggested 2TB; CPU: 8+ logical cores at 2+ GHz; OS: Windows (7 or newer; Virtualization (VT-x) needs to be enabled in the BIOS) or Unix (any flavour supporting Docker CE).

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