The AML1 component consists of a 156kb probe, labeled in red, located centromeric to the AML1 (RUNX1) gene that spans the CLIC6 gene and a 169kb probe covering part of the AML1 (RUNX1) gene, including markers SHGC-87606 and D21S1921. The ETO (RUNX1T1) component, labeled in green, consists of a 151kb probe covering the centromeric part of the gene and the flanking region and a 194kb probe covering the telomeric part of the gene and the flanking region.
I first came across CytoCell FISH probes in a previous lab I worked in and I was struck by the quality of the products. Since this time, I have been recommending and introducing CytoCell probes across all application areas — now they are the primary FISH probes used in our lab. They have an excellent range of products and their ready-to-use reagent format saves considerable time.
Medical Technologist, University of Arizona Health Network, USA