Array white papers and applications notes
Browse our collection of white papers and application notes for arrays.
The impact of microarray probe design on detecting copy number variants at exon-resolution
The analysis of structural variants, such as copy number variants (CNVs), is an important aspect of clinical genetics research. Whilst many technologies are used for determining CNVs in human DNA, array comparative genomic hybridisation (aCGH) is now established as the gold standard for detection of CNVs across the entire genome, and is used not just in research but also in clinical applications1.
CytoSure™ arrays offer robust copy-number detection with high resolution – detecting CNVs of a few hundred base pairs at the single-exon level. However, a number of different factors need to be taken into account when designing microarrays to make sure that they offer robust performance across the targeted regions. Learn more about the process that we undertake to make sure our arrays perform the best they possibly can.
1. Kearny, H.M. et al (2011). Genome in Medicine 13 p676-685
The use of the InnoScan® 710 scanner and Mapix® software with CytoSure microarrays
The InnoScan 710 scanner from Innopys is a widely used, low cost, portable microarray scanner capable of scanning microarray slides with a resolution of 3 µm. It is the fastest scanner on the market with an adjustable reading speed from 10 to 35 lines per second. Offering reliable and traceable results, with a barcode reader, InnoScan 710 provides accurate control of acquired images and is supplied with a validation slide and associated Mapix® software that verifies scanner performance as well as offering a sophisticated feature extraction function.
With two high performance lasers with an excitation of 532nm and 635nm, the InnoScan 710 scanner complements OGT’s CytoSure range of microarray slides, reagents and analysis software. This application note illustrates the use of the InnoScan 710 scanner and Mapix with a range of OGT CytoSure cytogenetic array formats.
Superior detection of chromosomal aberrations using the latest generation of exon-focused constitutional arrays
A vital aspect of clinical genetics research is the accurate and reliable detection of genetic aberrations, such as copy number variation (CNV) and loss of heterozygosity (LOH), particularly in the study of developmental disorders. Next generation sequencing (NGS) technology struggles to detect structural aberrations reliably and, as such, array comparative genomic hybridisation (aCGH) remains the gold standard1 for CNV detection.
This whitepaper from Dominic McMullan, West Midlands Regional Genetics Laboratory, Birmingham, UK, demonstrates that the CytoSure Constitutional v3 microarray can detect variants not detectable by other microarray platforms.
1. Curtis, C. et al (2009) The pitfalls of platform comparison: DNA copy number array technologies assessed. BMC Genomics 10, 588–610
Adoption of the CytoSure Constitutional v3 microarray for increased detection of disease-relevant variants
Even when considering the emergence of next generation sequencing technologies, array comparative genomic hybridisation (aCGH) remains a staple of the modern clinical genetics research laboratory, detecting with ease and confidence the genetic aberrations linked to many developmental disorders...
In this white paper, Kris Van Den Bogaert describes how this latest microarray technology is set to increase the detection of disease-relevant variants following a validation and implementation programme at the Department of Human Genetics, University Hospitals Leuven, Belgium.
Examining the medical exome - The advantages of customisable array designs for copy number variant detection in medical research
In light of recent advances in DNA analysis technologies, we are becoming ever more aware of the underlying complexity of genetic disorders. It is becoming increasingly evident that such disorders are rarely caused by an isolated mutation within a single gene, and one or many genes can contribute to one or multiple disorders. Moreover, causal aberrations vary widely from larger copy number variations (CNVs), down to single-point mutations...
Genetic diseases are complex and require an analytical approach that is both comprehensive and flexible. In this white paper, genetic scientists Dr Tracey Lewis and Dr Emily Farrow discuss how customisable, exon focused array designs complement next generation sequencing (NGS) for clinical genetic research.
Using the CytoSure Embryo Screen array to identify aneuploidies and large structural imbalances in Polar Bodies 1 and 2
Chromosome abnormalities are extremely common in human embryos particularly those generated during in vitro fertilisation (IVF). Embryos containing the wrong number of chromosomes (aneuploidy) or chromosomes with large structural abnormalities are known to be a major factor of pregnancy failure...
In this white paper learn how a team from Reprogenetics Germany and the MVZ Fertility Center, Hamburg have demonstrated the effective use of polar body screening as a method to detect large chromosomal abnormalities and show how parallel processing of 14 samples on one slide offers benefits to high-throughput laboratories over more traditional BAC arrays.
Evaluating and implementing CytoSure microarrays
With the speed of technological advances in the field of genetics, researchers can gain a host of benefits by continuing to consider the best options available, utilising the latest developments to enhance their work. Switching array platforms, however, might seem both daunting and potentially risky, and yet this can yield many advantages, improving efficiency and generating more insightful data in the most costeffective manner possible....
In this white paper learn how adopting the highest quality microarray technology is far easier than might be expected, following the results of the UK’s National Health Service tender.
CytoSure™: For research use only; not for use in diagnostic procedures.