NGS Success - Running the sequencer

View next generation sequencing (NGS) tips for running the sequencer:

  • Prepare Sequencing Pool: Use pooling worksheet to sequence equimolar amounts of libraries for sequencing based on laboratory recommendation.
  • If the sample sheet is incorrectly prepared, demultiplexing of your samples will not be carried out accurately. This would lead to difficulties in data analysis.
  • Prepare sample sheet: make sure the assay is single-index, paired end sequencing and use the proper setting from the sequencer to verify sample sheet parameters. If the sample sheet is incorrectly prepared, demultiplexing of your samples will not be carried out accurately. This would lead to difficulties in data analysis.

 

SureSeq™: For research use only; not for use in diagnostic procedures.