NGS Success - PCR1 Optimisation

View next generation sequencing (NGS) tips for PCR1 optimisation:

  • After adaptor ligation, use the lowest cycle number possible based on the DNA concentration. This will minimise duplication rates in sequencing results.

Fig. 1. Analysis of amplified prepared library DNA

Figure 1: Analysis of amplified prepared library DNA using an Agilent D1000 ScreenTape assay. The electropherogram shows a single peak in the size range of 200 to 275 bp (+/-10%).

 

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