NGS Success - PCR1 Optimisation
View next generation sequencing (NGS) tips for PCR1 optimisation:
- After adaptor ligation, use the lowest cycle number possible based on the DNA concentration. This will minimise duplication rates in sequencing results.
Figure 1: Analysis of amplified prepared library DNA using an Agilent D1000 ScreenTape assay. The electropherogram shows a single peak in the size range of 200 to 275 bp (+/-10%).