NGS Success - Hybridisation Temperature Optimisation

View next generation sequencing (NGS) tips for the hybridisation temperature optimisation:

  • Washes must be pre-warmed as per the handbook. 

Fig. 1. Comparison of Hybridisation Wash temperatures Figure 1. Comparison of Hybridisation Wash temperatures showing minimum coverage over the 7 gene targets in the Ovarian panel. Washing at  40˚C gives the best results.


SureSeq™: For research use only; not for use in diagnostic procedures.