NGS Success - End Repair and 3' End 'A' Tailing

View next generation sequencing (NGS) tips for end repair and 3' end 'A' tailing:

  • Prepare reaction on ice since enzyme has low activation temperature and will start prematurely at room temperature.
  • Samples must reach 4°C and be held at this temperature for 5-10 minutes before proceeding directly to adaptor ligation.

 

SureSeq™: For research use only; not for use in diagnostic procedures.