NGS Success - Adaptor Ligation

View next generation sequencing (NGS) tips for adaptor ligation:

Incubation

  • Prepare reaction on ice since the enzyme has a low activation temperature and will start prematurely at room temperature.
  • Use the proper master mix volume based on starting concentration.
  • Do not use the heated lid during incubation.

 

SureSeq™: For research use only; not for use in diagnostic procedures.