NGS Success

 

 

 

From pre-analytical QC to running the sequencer, this is the place to find the latest tips and advice from

our sequencing experts for all stages of the NGS protocol.

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The latest tip for NGS Success!

Mary Pierce-Burlingame, Field Application Scientist for SureSeq™ NGS

Check blanking reagent, be sure to use a similar pH and ionic strength as samples solutions to maximise Nanodrop accuracy.

View our collection of NGS tips for... 

  1. Pre-analytical QC
  2. All Purification Procedures
  3. DNA Shearing
  4. End repair and 3’ end ‘A’ Tailing
  5. Adaptor ligation
  6. PCR1 Optimisation
  7. Hybridisation Temperature Optimisation
  8. Preparing Magnetic Beads
  9. Hybrid Capture
  10. Washing Streptavidin Beads
  11. Post-Capture PCR
  12. Running the sequencer

 

SureSeq™: For research use only; not for use in diagnostic procedures.