NGS Success

 

 

 

From pre-analytical QC to running the sequencer, this is the place to find the latest tips and advice from

our sequencing experts for all stages of the NGS protocol.

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The latest tip for NGS Success!

Kicki tip

 

 

 

Be sure to prepare fresh 70% ETOH daily for washes. ETOH may evaporate over time and leave your mix at <70% ETOH. As ETOH evaporates, the concentration of your solution decreases. This may result in DNA being eluted from your Ampure XP beads and being discarded as waste supernatant. This may lead to the loss of important sample and experimental failure.

View our collection of NGS tips for... 

  1. Pre-analytical QC
  2. All Purification Procedures
  3. DNA Shearing
  4. End repair and 3’ end ‘A’ Tailing
  5. Adaptor ligation
  6. PCR1 Optimisation
  7. Hybridisation Temperature Optimisation
  8. Preparing Magnetic Beads
  9. Hybrid Capture
  10. Washing Streptavidin Beads
  11. Post-Capture PCR
  12. Running the sequencer

 

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