Array Advice - Quantitation
View tips and solutions to common problems for sample quantitation.
- Quantitate your gDNA using a Nanodrop spectrophotometer or a fluorometer e.g. Qubit.
- Spectrophotometers can over estimate the amount of DNA. For lower concentrations, use a fluorescent assay in addition to spectrophotometer (e.g. Qubit).
Solutions to Common Problems
Problem 1 - Inaccurate sample concentration
Cause: Sample concentration should be <400ng/ul. Inaccurate sample concentration can be caused because gDNA at high concentration cannot be quantitated accurately.
Solution: If the gDNA concentration is > 350 ng/μl, dilute 1:2 with water (or any of the recommended buffers).
Problem 2 - Precipitated DNA
Cause: Precipitated DNA can be caused by gDNA not being completely dissolved.
Solution: To resolve make sure that the gDNA is completely in solution by pipetting up and down. If needed, incubate at 37°C for 30 minutes.
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