Array Advice - Quantitation

View tips and solutions to common problems for sample quantitation.

Tips

  • Quantitate your gDNA using a Nanodrop spectrophotometer or a fluorometer e.g. Qubit.

Nanodrop and Qubit

  • Spectrophotometers can over estimate the amount of DNA. For lower concentrations, use a fluorescent assay in addition to spectrophotometer (e.g. Qubit).

Solutions to Common Problems

Problem 1 - Inaccurate sample concentration

Cause: Sample concentration should be <400ng/ul. Inaccurate sample concentration can be caused because gDNA at high concentration cannot be quantitated accurately. 

Solution: If the gDNA concentration is > 350 ng/μl, dilute 1:2 with water (or any of the recommended buffers).

 

Problem 2 - Precipitated DNA

Cause: Precipitated DNA can be caused by gDNA not being completely dissolved.

Solution: To resolve make sure that the gDNA is completely in solution by pipetting up and down. If needed, incubate at 37°C for 30 minutes.

 

CytoSure™ products are for research use only; not for use in diagnostic procedures