NGS Scientific Posters

Browse and view our collection of scientific posters for next generation sequencing (NGS).

The analysis of FFPE samples by next-generation sequencing (NGS) of key genes for research into breast and ovarian cancer

The analysis of FFPE samples by next-generation sequencing (NGS) of key genes for research into breast and ovarian cancer

Jacqueline Chan, Sabine Eckert, Lyudmila Georgieva and Graham Speight

Presented at AMP Europe 2018, this poster illustrates the confident detection of germline and somatic variants in key cancer-related genes including BRCA1 and TP53.

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The accurate detection by NGS of difficult to sequence genes (CALR, CEBPA, FLT3) associated with myeloid disorders using a hybridisation-based enrichment approach

The accurate detection by NGS of difficult to sequence genes (CALR, CEBPA, FLT3) associated with myeloid disorders using a hybridisation-based enrichment approach

Lyudmila Georgieva, Ezam Uddin, Jacqueline Chan, William Wright and Graham Speight

Presented at AMP 2017, this poster illustrates how the excellent coverage uniformity obtained using hybridisation-based enrichment and the SureSeq™ myPanel NGS Custom AML Panel can be successful in sequencing traditionally difficult genes.

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The assessment by NGS of FFPE derived tumour DNA using an ovarian cancer and a custom solid tumour hybridisation-based enrichment panel approach

The assessment by NGS of FFPE derived tumour DNA using an ovarian cancer and a custom solid tumour hybridisation-based enrichment panel approach

Jacqueline Chan, Juliette Forster, William Wright, Graham Speight

Presented at AMP 2017, this poster explains how the use of SureSeq hybridisation based panels in conjunction with the SureSeq FFPE Repair Mix provides superior uniformity of coverage compared to a PCR enrichment approach.

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The analysis of myeloproliferative neoplasm samples using a rapid (30 minute) hybridisation-based enrichment protocol for NGS

The analysis of myeloproliferative neoplasm samples using a rapid (30 minute) hybridisation-based enrichment protocol for NGS

Lyudmila Georgieva, Ezam Uddin, Jacqueline Chan and Graham Speight

Presented at the CGC 2017 annual summer meeting in Denver, USA, this poster illustrates the excellent quality data generated by the OGT 1-day hybridisation-based SureSeq LPK protocol in combination with the SureSeq Core MPN Panel.

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The application of a hybridisation-based NGS enrichment panel for the analysis of key genes involved in ovarian and breast tumours using DNA from FFPE samples

The application of a hybridisation-based NGS enrichment panel for the analysis of key genes involved in ovarian and breast tumours using DNA from FFPE samples

Jacqueline Chan, Sabine Eckert, Lyudmila Georgieva and Graham Speight

Presented at the 2017 Cancer Genomics Consortium (CGC) annual summer meeting in Denver, USA, this poster illustrates the SureSeq hybridisation-based approach as a robust method for the identification of germline and somatic mutations in TP53BRCA1, and BRCA2 and how it is possible to obtain important sequence information from as little as 50 ng of formalin-compromised DNA.

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The application of a hybridisation-based NGS enrichment panel for the analysis of somatic variants in tumour samples and reference standards

The application of a hybridisation-based NGS enrichment panel for the analysis of somatic variants in tumour samples and reference standards

Jacqueline Chan , Lyudmila Georgieva , Sabine Eckert , Faidra Partheniou and Graham Speight

Presented at the 42nd Association of Genetic Technologists (AGT) 2017 annual meeting in St Louis, USA, this poster outlines the application of a hybridisation-based next-generation sequencing enrichment panel for the analysis of solid tumour somatic variants, demonstrating 100% concordance in variant detection in both genomic and formalin-compromised DNA and superior uniformity of coverage from hybridisation-based enrichment when compared to an amplicon-based method in key exons of BRCA1 and BRCA2.

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The application of a one-day hybridisation-based enrichment protocol for NGS incorporating a rapid (30 minute) hybridisation step

The application of a one-day hybridisation-based enrichment protocol for NGS incorporating a rapid (30 minute) hybridisation step

Lyudmila Georgieva, Ezam Uddin, Jacqueline Chan, Faidra Partheniou, and Graham Speight

Presented at the 42nd Association of Genetic Technologists (AGT) 2017 annual meeting in St Louis, USA, this poster outlines how OGT has optimised a one-day hybridisation-based enrichment protocol for next-generation sequencing (NGS) incorporating a rapid 30 hybridisation step, with performance examples illustrating excellent uniformity of coverage in the detection of key CALR and JAK2 indels (including 52 bp deletions and 5 bp insertions) and FLT3 ITDs.

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An approach for determination of copy number variation using short-read NGS

An approach for determination of copy number variation using short-read NGS

Kaajal Reeves , Mafalda Bourbon, Sandra Kachhia , Douglas Hurd , James Reid , Duarte Molha , Darren Houniet and John Cousin

Presented at the European Society of Human Genetics (ESHG) 2017 annual meeting in Copenhagen, Denmark, this poster outlines how OGT’s combined bioinformatics and focused panel approach offers the ability to analyse both germline copy number variation (CNV) and single nucleotide variation (SNV) using a single assay, with performance demonstrated across several samples in both the LDLR and DMD genes. All results were concordant with other techniques.

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Optimised, 1-day hybridisation-based NGS protocol yields 1% variant detection in MPN samples, as quickly and cost-effectively as multiplex PCR

Optimised, 1-day hybridisation-based NGS protocol yields 1% variant detection in MPN samples, as quickly and cost-effectively as multiplex PCR

Graham Speight , Ephrem Chin , Lyudmila Georgieva, Nick Cross and David Cook

Presented at the Association For Molecular Pathology (AMP) 2016 annual meeting in Charlotte, NC, USA in November 2016, this poster outlines how the SureSeq Core MPN Panel can accurately detect alleles down to 1% variant allele fraction (VAF) in JAK2 (V617F) at a read depth of >1000x, facilitating reliable detection, not only single nucleotide variants, but also 5 bp insertions in JAK2 (exon 12) and deletions of up to 52 bp in CALR (exon 9). 

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The use of a hybridisation-based NGS enrichment panel for the confident identification of a broad range of low frequency variants from as little as 50ng of challenging clinical research FFPE samples

The use of a hybridisation-based NGS enrichment panel for the confident identification of a broad range of low frequency variants from as little as 50ng of challenging clinical research FFPE samples

Graham Speight, Ephrem Chin, Jacqueline Chan, Robert Zeillinger, Nicole Concin, David Cook

Presented at the Association For Molecular Pathology (AMP) 2016 annual meeting in Charlotte, NC, USA in November 2016, this poster outlines how the SureSeq FFPE DNA Repair Mix significantly improves NGS library yields, with an increase of mean target coverage (increased by >2.2 fold), resulting in more meaningful data and therefore confident variant calling from this challenging sample type.

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SureSeq™: For research use only; not for use in diagnostic procedures.