FISH application notes and customer interviews

Browse our collection of application notes and customer interviews for fluorescence in situ hybridisation (FISH).

Digesting the indigestible - Rescuing FISH on FFPE

Customer interview: Digesting the indigestible - Rescuing FISH on FFPE

OGT’s Cytocell® Tissue Pretreatment Kit uses optimised, ready-to-use reagents in a simple two-stage FFPE slide preparation protocol to increase  the permeability of cell membranes and facilitate penetration of FISH or CISH DNA probes.

We spoke to Carmen Vokaty, Assistant-Chief Medical Technologist in Cytogenetics at the McGill University Health Centre, about how her lab incorporated the kit into its workflow after struggling with a homebrew solution. She explains how the kit revolutionised her lab’s pretreatment process and the quality of FISH results, enabling the lab to save time, increase capacity and dramatically reduce the need to repeat samples, if any non-retrievable tissue were still available.

Read and download the application note >

(Please note - you will view this poster on our Cytocell FISH probes website)

 

Validation of signal pattern cut-off levels

App note: Validation of signal pattern cut-off levels

In December 2018 Oxford Gene Technology received FDA clearance for eight Cytocell Aquarius® fluorescence in situ hybridisation (FISH) probes.

These kits are FISH tests used to detect common chromosomal rearrangements found in 3:1 methanol/acetic acid fixed bone marrow specimens from patients with AML and MDS.

In order to fulfil the high standards required for an FDA clearance, all eight Cytocell FISH probes in this submission underwent stringent performance assessments to underpin the probe performance and ensure that the products were safe and effective for their intended use.

This application note provides customers with information on validation of signal pattern cut-off levels when using Cytocell Aquarius FDA-cleared AML-MDS probes.

Read and download the application note >

(Please note - you will view this poster on our Cytocell FISH probes website)

 

Under the microscope - Saving time and costs in FISH automation

Customer interview: Under the microscope - Saving time and costs in FISH automation

With the demand for FISH (fluorescence in situ hybridisation) testing on the rise in many pathology labs, automating part or all of the workflow can help to ease time and cost burdens. Many labs choose to automate slide processing to achieve this, but these systems also come with some restrictions. Manufacturers typically pre-validate a limited choice of FISH probes and there is uncertainty whether other vendor’s probes can be successfully used, without complicated validation processes.

Dr Jennie Thurston, Director of Cytogenetics, Carolinas Pathology Group, Atrium Health, spoke to us about how her lab incorporated a slide processor into their workflow to address their ever-increasing sample workload. She explains how Oxford Gene Technology’s (OGT’s) Cytocell FISH probes were seamlessly incorporated into the automated workflow for fast, high quality results. Dr Thurston also shares the benefits and experience gained by her lab as well as her insight on the future of FISH testing in pathology laboratories.

Read and download the application note >

(Please note - you will view this poster on our Cytocell FISH probes website)

 

Successful Cytocell FISH probe adoption into an automated workflow

App note: Successful Cytocell FISH probe adoption into an automated workflow

Many laboratories prefer the flexibility to choose FISH probes from alternative suppliers rather than those provided by the instrument manufacturer. However, the biggest challenges when attempting to implement a new FISH probe into an automated workflow are the time and expense required to optimise each one.

In this application note, Dr. Jennie Thurston from Carolinas Pathology Group, North Carolina, USA, demonstrates the use of Cytocell FISH probes with FFPE cell line samples prepared using the Abbott Molecular VP2000 Processor (50/60Hz CE IVD).

This study demonstrates that no further optimisation of the current laboratory FISH protocol or VP2000 workflow is required when using Cytocell FISH probes.

Read and download the application note >

(Please note - you will view this poster on our Cytocell FISH probes website)

 

Application Note - Reducing your FISH probe validation burden

App note: Reducing your FISH probe validation burden

This application note describes the detailed clinical and analytical studies carried out to meet the performance required to achieve FDA clearance of the eight probes for acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS).

In addition, we present data demonstrating excellent analytical reproducibility of the probes and extensive stability studies.

During the course of these studies, which spanned four sites, worldwide, over 2,500 replicates were run with no technical failures. The rigorous standards required for gaining FDA clearance are recognized globally and emphasize the exceptional quality of the Cytocell FISH probes from Oxford Gene Technology (OGT).

Read and download the application note >

(Please note - you will view this poster on our Cytocell FISH probes website)

 

Disclaimer

GlobalmyProbes®: For Research Use Only; Not for Use in Diagnostic Procedures. Cytocell®: Product availability may vary from country to country and is subject to varying regulatory requirements. Please contact your local representatives for availability.

USA: Cytocell Aquarius® AML/MDS range of FISH probe kits: Refer to individual test kit Package Insert for the specific intended use and limitations. For In Vitro Diagnostic Use. Rx only. Other Cytocell® and myProbes® FISH probes: Analyte Specific Reagent. Analytical and performance characteristics are not established. Product availability may vary from country to country and is subject to varying regulatory requirements. Please contact your local representatives for availability.