Enhanced bait design strategies that deliver more reliable NGS panel assays

Friday 22 May 2015
Applications:

Enhanced bait design strategies that deliver more reliable NGS panel assays

As next generation sequencing (NGS) moves closer to the clinic, especially in the field of cancer detection, it is becoming apparent that the choice of target enrichment methodology can have a dramatic effect on the ability to reliably detect all loci exhibiting disease-associated mutations. Only the most reproducible detection methods will inspire enough confidence to allow the transfer of NGS assays from a useful research tool to a clinically dependable one. While many enrichment approaches generate highly amplified target DNA, there can be issues with PCR artefacts, excessive off-target enrichment, target region bias and non-uniformity of coverage or even wholesale target region drop-out.

In this latest webinar, Chris Mattocks, Senior Clinical Research Scientist at the National Genetics Reference Laboratory (Wessex), Salisbury, UK, will discuss the advantages of hybridisation enrichment and the importance of optimal bait design to ensure increased coverage uniformity for all target regions