Evaluation of DNA labelling kits for enhanced microarray results

Friday 21 March 2014
Product types:

Ruth Burton and Douglas Hurd

Introduction

Array comparative genomic hybridisation (aCGH) is a well-established technique used to detect chromosomal abnormalities, particularly in conditions such as development delay and intellectual disabilities. To identify potentially disease causative genomic regions, DNA from the test sample is labelled with one fluorescent dye, usually Cyanine 5 and a second reference sample is labelled with a different fluorescent dye, typically Cyanine 3. The two labelled samples are then competitively hybridised to a microarray. Differences in copy number between the test and reference sample are calculated by measuring the signal intensities of the two dyes and converting these measurements to a ratio. Efficient labelling of both test and reference samples is a critical step in the microarray process and poor labelling can result in inaccurate and potentially misleading data.

This application note provides a technical evaluation of CytoSure™ Genomic DNA Labelling Kits compared with another leading DNA labelling kit. CytoSure Genomic DNA Labelling Kits are shown to improve key microarray quality control (QC) metrics and consistently generate data with improved signal-to-noise ratios and low derivative log ratio spreads (DLRS).

Our lab has tried a variety of different labelling methods; and found OGT’s CytoSure Genomic DNA Labelling Kit to give consistently low DLRS values and excellent signal-to-noise ratios. Cytogenetics Laboratory, Baylor College of Medicine

 

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