Next generation sequencing survey 2013

Tuesday 20 August 2013

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596 researchers working with NGS responded to our survey to find out their current use and future demands from the market — with interesting results. For each survey completed, a donation was made to DEBRA (UK charity for the genetic skin blistering condition Epidermolysis Bullosa).

Response rate (%) per country                                                  Type of organisation

Response rate (%) per country

Type of organisation












The majority of the respondents are located in the UK and US working in an academic orgaisation.


Most popular NGS methods- your top choices


Most popular NGS methods



Targeted resequencing (i.e., targeted panel sequencing and exome sequencing) and RNA-seq are in high demand with 72% of respondents planning to use one of these methods in the next 12 months. Only 13% of respondents are interested in data analysis-intensive whole genome sequencing.




Top research areas for NGS


Top research areas for NGS




Tying in with the interest in targeted resequencing, cancer and rare disease are the most popular research areas for NGS. Offering cost-effective, highresolution analysis, targeted resequencing is a logical choice for these research areas, enabling accurate detection of rare variants.

Higher resolution also increases data accuracy and confidence, which is particularly important when developing a clinical test. This is likely to be a primary reason why over 60% of respondents look to use targeted panel resequencing for such assays.


Your preferred sequencing platform for targeted panel sequencing


Illumina platforms are revealed to be the market-leading sequencing technology for targeted panel sequencing.*

Your preferred sequencing platform for targeted panel sequencing













*Respondents were allowed multiple answers, which is why the figures do not total 100%



Barriers to NGS usage


Barriers to NGS usage


When asked reasons why NGS might be difficult to implement in their investigations, researchers cited bioinformatics, including data analysis and interpretation as the biggest challenges, followed by test parameters such as experimental design and sample quality.

As a consequence, over a third of researchers are choosing to outsource their data analysis to dedicated service providers for rapid access to the meaningful results they need.

36% are outsourcing data analysis



What researchers want from NGS service providers

The top 3 essential factors when choosing an NGS service provider highlight a strong need for quality metrics, expert data analysis and fast delivery of results:

• Confidence in data quality 38%

• Bioinformatics expertise for rapid data analysis 22%

• Rapid turnaround time 13%



About OGT

Oxford Gene Technology (OGT) is a leading genomic services provider offering flexible, comprehensive next generation whole exome, pre-designed panel, or custom panel sequencing. OGT Genefficiency™ Genomic Services provide complete management of the process from fully optimised project and capture design through to the delivery of an intuitive, user-friendly report, providing investigators with easy access to meaningful data — without the need for in-house bioinformatics resource.

For more information about OGT’s Genefficiency Sequencing Services visit or email

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