Custom Genomic Research Solutions

Friday 18 May 2018
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Specialists for customisation

Oxford Gene Technology (OGT) provides world-class genetic research solutions for detecting the causative variations underlying genetic disease. We are committed to the field of molecular genetics and provide our customers with the most advanced tools available, backed by exceptional technical support.

Our experience and expertise are also the foundation of our custom products portfolio. Custom NGS panels, custom arrays and custom FISH probes give researchers the ability to react to new discoveries, modify research workflows, and target specific regions in a fast-changing genomics research environment.

Each custom project from OGT is designed with the help of an in-house team of experts, dedicated to your project from design to delivery. Quality is assured and getting started could not be simpler.

myProbes custom Cytocell FISH probes

 

 

 

 

 

Custom FISH probes may range from a simple catalogue probe modification to a truly unique product. Are you studying a rare gene and can’t find a commercially available FISH probe? We can help.

myProbes is a custom design and manufacture service that provides unique fluorescence in situ hybridisation (FISH) probes using the BAC-2-FISH™ process. This process utilises Cytocell’s proprietary BAC clone collection containing >220,000 clones to produce fully quality-assured custom FISH probes for virtually any sequence in the entire human genome.

  • Completely unique product
  • No setup fee
  • Excellent results - Quality-assured FISH probes are manufactured under GMP and GLP guidelines and made under a quality system certified to ISO13485:2003 and ISO9001:2008 quality standard

We used to make our own probes when they weren’t available commercially, but this was a really tough and lengthy process. It has been really helpful to be able to hand over that work to the Cytocell myProbes team who always deliver us with great performing probes. Claire Schwab, Research Cytogeneticist at the Wolfson Childhood Cancer Research Centre, Newcastle University

Expert consultation available from start to finish

To get started on a myProbes custom FISH probe project, simply follow these steps and our in-house team of experts does the rest.

Follow these steps and our in-house team of experts does the rest

We have completed evaluation and validation on a number of probes designed by the myProbes team and love the performance. Professor Mark Pettenati, Cytogenetics and Molecular Cytogenetics, Wake Forest University Medical Center, North Carolina, USA

Learn more about Cytocell custom probes

 

CytoSure Custom Designed CGH Arrays

 

 

 

 

 

 

Create a custom CGH array with Oxford Gene Technology and work with the world leaders in microarray design. Our proprietary Oligome™ database contains more than 26 million oligonucleotide probes, either in silico or empirically optimised, to ensure the best possible performance and minimum noise across any desired genomic targets.

CytoSure custom arrays are optimised using our exclusive microarray design pipeline and enable you to target your exact specifications. With a number of different formats available, every lab’s throughput and cost requirements can be met.

  • Accurate detection of copy number variation using the gold-standard technology
  • Streamlined workflows for analysis of many genes simultaneously
  • Flexible format - 1, 2, 4, or 8 arrays available per slide to provide the most cost-effective solution
  • Streamlined data analysis using industry-leading CytoSure Interpret™ software

All custom array projects are managed by experienced bioinformaticians who, after extensive consultation, will create your bespoke array. The array can be designed against any fully or partially sequenced genome as well as against sequencing data.

In addition, OGT has extensively research-validated SNP content for detection of uniparental disomy (UPD) and loss of heterozygosity (LOH), which can be incorporated into the array design.

Pick and choose from existing, optimised content

All of our pre-optimised, proven and popular designs — such as the selection below — are available for customisation. Take your pick from existing content, or design your own.

Autism; cancer; cardiomyopathy; ciliopathies; epilepsy; eye disease; metabolic disorders; neuromuscular disease (NMD); skeletal dysplasia

To aid in distinguishing the presence of a processed pseudogene from a true gene duplication, we required additional probes in the intronic regions of SMAD4. OGT were quick to respond to our requests with the design of additional probes, and the ability to work closely with the OGT design team to optimise array content is a great advantage for us. Tracey Lewis, Research Scientist in the R&D Molecular Genomics Group, ARUP Laboratories, Salt Lake City

Optimised arrays show improved results

OGT’s extensive array design work and empirical optimisation process results in better probe specificity, reduced noise, and more accurate results. This example shows separate experiments on the same sample with a confirmed ~1600 bp deletion.

Experiment run using nonoptimised array, with an inaccurate ~400 bp deletion detected.

Experiment run using nonoptimised array, with an inaccurate ~400 bp deletion detected.

Experiment run using optimised array, with an accurate ~1600 bp deletion detected.

Experiment run using optimised array, with an accurate ~1600 bp deletion detected.

A streamlined custom design process

Take the burden out of customisation by using OGT’s custom design process. From your specification, we develop highly optimised products ready to provide unparalleled data quality. As new requirements arise, array designs are easily updated.

From your specification, we develop highly optimised products ready to provide unparalleled data quality

Learn more about our custom designed aCGH arrays

 

SureSeq myPanel custom NGS panels

 

 

 

 

 

 

Simply select the gene, exonic or intronic content you need from OGT’s regularly updated, expert-curated library of pre-optimised NGS panel content and sequence only the genes you are interested in.

OGT’s unique panel design coupled with hybridisation-based enrichment offers unparalleled coverage completeness and uniformity. This allows the accurate detection of difficult to sequence, low-frequency variants and minimises the requirement for supplementary fill-in with Sanger sequencing.

  • Accurate detection of low-frequency variants
  • Panels are pre-optimised to work with your samples, eliminating laborious in-house optimisation and decreasing assay development time
  • Panel content designed with experts and from current literature to target all relevant regions including intronic and splice sites

Working with OGT has been very positive, we’ve been happy to collaborate with them and this has made it easy to customise their products. Dr Anna Skowronska (R&D scientist), West Midlands Regional Genetics Laboratory (WMRGL) of the Birmingham Women’s NHS Foundation Trust, UK

Choose your genes of interest

Pre-optimised NGS custom panel content is available for research into a wide range of conditions, including:

AML; breast cancer; CLL; CML; colorectal cancer; Familial Hypercholesterolemia; glioma; lung cancer; melanoma; Myelodysplastic Syndromes; Myeloproliferative Neoplasms; Ovarian Cancer; Prostate Cancer; Sarcoma

Excellent coverage uniformity

Mutations in the CEBPA gene are among the most common molecular alterations in AML. Sequencing of the CEBPA gene is often hampered by a repetitive nucleotide sequence and a high GC-rich content, which can lead to technical challenges in assay design, requiring a supplementary fill-in with Sanger sequencing. OGT’s expert bait design overcomes these issues, to offer a high level of uniform coverage for a notoriously difficult gene to sequence in research applications.

Illustration of the excellent uniformity of coverage of the CEBPA gene

Illustration of the excellent uniformity of coverage of the CEBPA gene averaging ~3000x coverage. Depth of coverage per base (grey). Targeted region (green). Gene coding region as defined by RefSeq (blue). GC percentage (red). Repeat regions, and those rich in GC (pink).

Getting started could not be easier

SureSeq myPanel workflow

Learn more about our custom NGS panels

 

CytoSure, SureSeq and myProbes: For Research Use Only; Not for Use in Diagnostic Procedures. Cytocell: Some products may not be available in the US.

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Do you have a question about what you've just read? Contact us today and one of our technical specialists will be happy to answer any questions you may have.

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