Evaluation of enzymatic DNA digestion as an alternative to mechanical DNA fragmentation (sonication) for targeted NGS using the SureSeq™ Myeloid Panel

Tuesday 7 June 2016
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Introduction

DNA fragmentation is a crucial first step in the preparation of libraries for next generation sequencing (NGS). Mechanical shearing (sonication) is currently the gold-standard for fragmentation but it requires a significant upfront capital investment. Oxford Gene Technology (OGT) has evaluated an alternative method of fragmentation using the NEBNext® dsDNA Fragmentase®.

 

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