Increasing numbers of aCGH samples combined with higher-throughput array formats means that it is imperative to track samples throughout the labelling, hybridisation and analysis process to maintain sample identity. CytoSure® Sample Tracking Spike-ins are uniquely designed to enable reliable sample tracking and easy identification of sample mix-up using OGT’s classleading CytoSure Arrays and CytoSure Interpret Software.
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As aCGH is now recognised as the first-tier test for research into numerous cytogenetic aberrations, many laboratories are scaling up their processes to increase throughput and reduce costs. Parallel processing of higher numbers of samples increases the possibility of sample mix-up. Even automated workflows contain several steps where sample identity can be lost (e.g. pipetting samples into gasket slides). CytoSure Sample Tracking Spike-ins enable researchers to quickly and easily identify any erroneous samples ensuring only accurate data is reported.
Each CytoSure Sample Tracking Spike-in is designed to a specific, unique region of the genome. Oligonucleotide probes complementary to the spike-ins are included on all of the arrays supplied and optimised by OGT. Eight different CytoSure Sample Tracking Spike-ins are available. Each spike-in has been carefully prepared to ensure that there is no cross-hybridisation with other probes on the array or with any other region on the genome. Each spike-in supplied is sufficient for 12 labelling reactions and is provided in 3 separate aliquots to avoid potential DNA degradation caused by repeated freeze thawing. In addition, colour-coded caps are used for ease of identification, aiding correct usage. The spike-in is pipetted into the labelling reaction, then the labelling reaction and array processing continues following the standard workflow. CytoSure Sample Tracking Spike-ins are particularly suitable for use when labelling reactions are prepared in tubes and ideally complement the 24 reaction CytoSure Genomic DNA Labelling Kit.
The specific CytoSure Sample Tracking Spike-in used is simply selected in the CytoSure Interpret Software and the system automatically confirms if the resultant data is correct for this spike-in, thereby identifying whether there has been a sample mix-up (Figure 1).
Figure 1: Fast and intuitive entry of CytoSure Sample Tracking Spike-in into CytoSure Interpret Software. Drop-down menus allow the user to quickly enter the specific spike-in used in the labelling reaction. The identity of the sample tracking spike-in is clearly displayed after analysis.
I would definitely recommend OGT to any colleague. The platform has been easy to transfer to, improving our processes, and we’re very happy using it within our area of developmental delay. If anyone ever asks me if it was worth the effort of transferring to OGT, the answer is a clear yes.
Kath Smith
Consultant Clinical Scientist, Sheffield Children's NHS Foundation Trust
