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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest CLL
  • Region 6q23.3 / 6p11.1-q11.1
    11q22.3 / 11q13.3 / 11q12
    12p11.1-q11.1
    13q14.2-q14.3 / 13q34
    14q32.33
    17p13.1 / 17p11.1-q11.1
    18q21.33
  • Label    
  • Product No. RU-PMP 016 (10 devices)
    RU-PMP 017 (5 devices)
    RU-PMP 018 (2 devices)
    RU-PMP 020 (contains x4 PMP 017)
  • Intended use For research use only; not for diagnostic procedures.

Overview

The Multiprobe system is an extension of CytoCell’s proprietary Chromoprobe® technology whereby DNA FISH probes are reversibly bound to the surface of a glass device. This system allows multiple FISH probes to be hybridised on the same slide in a spatially separated manner.

The Multiprobe CLL Panel (RUO) has been designed to detect up to eight different FISH probes on a single slide in a single hybridisation experiment. The assay is supplied in a kit format of 2, 5, 10 or 20 devices and includes hybridisation solution, DAPI counterstain, template slides, a hybridisation chamber and full instructions for use. The kit even contains a unique liquid crystal display slide surface thermometer for accurate temperature measurement of the denaturation surface.

Probe orientation

1

MYB Deletion

2

Chromosome 12 Alpha Satellite

3

ATM Deletion

4

IGH/BCL2 Translocation, Dual Fusion

5

IGH Breakapart

6

P53 Deletion

7

IGH/CCND1 Translocation, Dual Fusion

8

13q14.3 Deletion

Probes

MYB Deletion

Probe specification

  • MYB, 6q23.3, Red
  • D6Z1, 6p11.1-q11.1, Green

The MYB probe is 183kb in length and is labelled in red. It covers the MYB gene and continues into the centromeric region of the AHI1 gene beyond marker AFMA074ZG9. The probe mix also contains a control probe for the 6 centromere (D6Z1) labelled in green.

Chromosome 12 Alpha Satellite

Probe specification

  • D12Z3, 12p11.1-q11.1, Red

The Chromosome 12 Alpha Satellite Probe is a repeat sequence probe, labelled in red, which recognises the centromeric repeat sequence D12Z3.

ATM Deletion

Probe specification

  • ATM, 11q22.3, Red
  • 11q12.1, Green

The ATM probe is 182kb in length and is labelled in red. It covers the telomeric end of the NPAT gene and the centromeric end of the ATM gene to beyond the D11S3347 marker. The probe mix also contains a 11q12.1 control probe labelled in green, covering a 122kb region including the SHGC-154780 marker and a 130kb region including the SHGC-110550 marker.

IGH/BCL2 Translocation, Dual Fusion

Probe specification

  • IGH, 14q32.3 Green
  • BCL2, 18q21.3 Red

The IGH/BCL2 product consists of probes, labelled in green, covering the Constant, J, D and Variable segments of the IGH gene, and a 585kb probe, labelled in red, covering the BCL2 and KDSR genes.

IGH Breakapart

Probe specification

  • IGHC, 14q32.3, Red
  • IGHV, 14q32.3, Green

The IGH product consists of a 176kb probe, labelled in red, covering part of the Constant region of the gene and a green probe, covering part of the Variable segment of the gene.

P53 (TP53) Deletion

Probe specification

  • P53, 17p13, Red
  • D17Z1, 17p11.1-q11.1, Green

The P53 probe is 161kb in length and is labelled in red. It covers the whole P53 (TP53) gene and flanking regions. The probe mix also contains a control probe for the 17 centromere (D17Z1) labelled in green.

IGH/CCND1 Translocation, Dual Fusion

Probe specification

  • IGH, 14q32.3, Green
  • CCND1, 11q13.3, Red

The IGH/CCND1 product consists of probes, labelled in green, covering the Constant, J, D and Variable segments of the IGH gene, and CCND1 probes, labelled in red. The CCND1 probe mix contains a 155kb probe centromeric to CCND1 gene, covering a region between the D11S2663 and the D11S4095 markers, and a second probe (162kb) covering the telomeric end of CCND1 gene and the region up to FGF3 gene.

13q14.3 Deletion

Probe specification

  • 13q14.2-q14.3, Red
  • 13qter, 13q34, Green

The 13q14.3 probe, labelled in red, covers the D13S319 and D13S25 markers. The 13qter subtelomere specific probe (clone 163C9), labelled in green, allows identification of chromosome 13 and acts as a control probe.

Multiprobe procedure

Select a protocol step to view:

Step 1

Step 1 Thumbnail
  • Soak the slides in 100% methanol, then polish dry with a lint free cloth.

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