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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest AML, MDS
  • Region 5p15.31 / 5q31.2
    7q22.1-q22.2 / 7q31.2
    8q21.3
    11q23.3
    15q24.1
    16p13.11 / 16q22.1
    17q21.1-q21.2 / 17p13.1
    20q12 / 20q13.12
    21q22.12
  • Label    
  • Product No. RU-PMP 025 (2 devices)
    RU-PMP 026 (5 devices)
    RU-PMP 027 (10 devices)
    RU-PMP 028 (contains x4 PMP 026)
  • Intended use For research use only; not for diagnostic procedures.

Overview

The Multiprobe system is an extension of CytoCell’s proprietary Chromoprobe® technology whereby DNA FISH probes are reversibly bound to the surface of a glass device. This system allows multiple FISH probes to be hybridised on the same slide in a spatially separated manner.

The Multiprobe AML/MDS Panel (RUO) has been designed to detect up to eight different FISH probes on a single slide in a single hybridisation experiment. The assay is supplied in a kit format of 2, 5, 10 or 20 devices and includes hybridisation solution, DAPI counterstain, template slides, a hybridisation chamber and full instructions for use. The kit even contains a unique liquid crystal display slide surface thermometer for accurate temperature measurement of the denaturation surface.

Probe orientation

1

Del(5q) Deletion

2

PML/RARα Translocation, Dual Fusion

3

P53 Deletion

4

AML1/ETO Translocation, Dual Fusion

5

MLL Breakapart

6

Del(7q) Deletion

7

CBFβ/MYH11 Translocation, Dual Fusion

8

Del(20q) Deletion

Probe specification

Del(5q) Deletion

Probe specification

  • EGR1, 5q31.2, Red
  • 5p15.3, Green

The EGR1 probe, labelled in red, covers a 186kb region within 5q31.2, including the D5S500 marker. The probe mix also contains a control probe for chromosome 5 at 5p15.3, including the marker D5S630, labelled in green.

PML/RARα (RARA) Translocation, Dual Fusion

Probe specification

  • PML, 15q24.1, Red
  • RARα, 17q21.1-q21.2, Green

The PML probe mix, labelled in red, consists of a 151kb probe centromeric to the PML gene and a 174kb probe telomeric to the PML gene. The RARα probe mix, labelled in green, consists of a 167kb probe centromeric to the RARα (RARA) gene, including the CASC3 gene, and a 164kb probe, including the telomeric end of the RARα gene as well as the TOP2A and IGFBP4 genes.

P53 (TP53) Deletion

Probe specification

  • P53, 17p13, Red
  • D17Z1, 17p11.1-q11.1, Green

The P53 probe is 161kb in length and is labelled in red. It covers the whole P53 (TP53) gene and flanking regions. The probe mix also contains a control probe for the 17 centromere (D17Z1) labelled in green.

AML1 (RUNX1)/ETO (RUNX1T1) Translocation, Dual Fusion

Probe specification

  • AML1, 21q22.1, Red
  • ETO, 8q21.3, Green

The AML1 probe mix, labelled in red, consists of a 156kb probe centromeric to the AML1 (RUNX1) gene, including the CLIC6 gene and a 169kb probe covering part of the AML1 (RUNX1) gene and extending beyond the marker D21S1921. The ETO probe mix, labelled in green, consists of two probes, 151kb and 194kb, on either side of the ETO (RUNX1T1) gene.

Chromosome 8

The Multiprobe AML/MDS Panel (RUO) has been designed so that chromosome 8 can be enumerated using the ETO probe on square 4.

MLL (KMT2A) Breakapart

Probe specification

  • MLL, 11q23.3, Red
  • MLL, 11q23.3, Green

The MLL product consists of an 87kb probe, labelled in red, covering a region telomeric to the MLL (KMT2A) gene including the marker SHGC-111513 and a green probe covering a 170kb region centromeric to the MLL gene spanning the CD3G and UBE4A genes.

Del(7q) Deletion

Probe specification

  • 7q22. Red
  • 7q31.2, Green

The 7q22 probe, labelled in red, covers a 396kb region including the telomeric end of the RELN gene and extending beyond the marker D7S658. The 7q31.2 probe, labelled in green, covers a 203kb region including the TES gene.

CBFβ (CBFB)/MYH11 Translocation, Dual Fusion

Probe specification

  • CBFβ, 16q22, Red
  • MYH11, 16p13.1, Green

The CBFβ probe, labelled in red, covers a 617kb region within 16q22 and includes the CBFβ gene. The MYH11 probe, labelled in green, covers a 621kb region within 16p13.1 and includes the MYH11 gene.

Del(20q) Deletion

Probe specification

  • 20q12, Red
  • 20q13.1, Green

The 20q12 probe, labelled in red, covers a 331kb region within the PTPRT gene and includes the D20S108 marker. The 20q13.1 probes, labelled in green (141kb and 174kb), cover the MYBL2 gene and include the D20S150 marker.

Multiprobe procedure

Select a protocol step to view:

Step 1

Step 1 Thumbnail
  • Soak the slides in 100% methanol, then polish dry with a lint free cloth.

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