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SureSeq™ Core MPN Panel

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The SureSeq Core MPN Panel delivers accurate detection of somatic variants in 3 clinically relevant myeloid-associated genes, JAK2, MPL and CALR.

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For more information about any OGT product or service, please contact us.

Email: contact@ogt.com
Phone: +44 (0)1865 856800 (US: 914 467 5285)

Details

The SureSeq Core MPN Panel has been designed by Oxford Gene Technology (OGT) in collaboration with recognised cancer experts to deliver accurate detection of somatic variants in 3 clinically relevant myeloid-associated genes, JAK2, MPL and CALR, for research into the diagnosis, aetiology and prognosis of MPN.

The SureSeq Core MPN Panel offers:

  • Hybridisation-based panel delivering unparalleled coverage uniformity — detect low frequency variants consistently down to 1% VAF at a read depth of >1000x with confidence
  • Time and cost saving solution — replace multiple single gene assays with a single NGS panel
  • Focused panel content — sequence only what’s relevant for your cancer research, increase throughput and save on sequencing reagents
  • Hybridisation quality — Amplicon speed – streamlined library preparation, rapid 30 minute hybridisation step, 1 day from sample to sequencer
  • Excellent coverage uniformity — >99% of targeted regions are covered to at least 50% of mean target coverage

Reliable Detection of SNVs, Insertions and Deletions

The SureSeq Core MPN Panel in combination with the enhanced library preparation workflow is able to reliably detect single nucleotide variants (SNVs) as well as insertions (5 bp insertion in JAK2 exon 12 and CALR exon 9) and deletions (5 bp deletion in JAK2 exon 12 and 52 bp deletion in CALR exon 9) (Figures 1 and 2).

Detection of a 52 bp deletion (exon 9 CALR)Figure 1: Detection of a 52 bp deletion (exon 9 CALR). Wild-type sample (top panel) is compared to a 52 bp somatic deletion (bottom panel).

Detection of a 5 bp deletion (exon 12 JAK2)

Figure 2: Detection of a 5 bp deletion (exon 12 JAK2). Wild-type sample (top panel) is compared to a 5 bp somatic deletion (bottom panel).

Panel Coverage

The panel covers sites of relevance in the following 3 genes:

Gene Exon Mutation
MPL
 10 W515
CALR 9 insertions/deletions
JAK2 12 insertions/deletions amino acid substitutions
JAK2 14 V617F

 

If you are looking for an extended myeloid panel, choose our SureSeq Myeloid Panel covering 25 genes of interest, or customise your own panel with our SureSeq myPanel service.

Ordering Information

Product Contents Cat. No. Price
SureSeq Core MPN Panel (16) Enrichment baits; sufficient for 16 samples; SureSeq Interpret Software 602002 Get a quote
SureSeq Core MPN Panel (96) Enrichment baits; sufficient for 96 samples; SureSeq Interpret Software 602001 Get a quote
SureSeq Myeloid Panel (16 reactions) Enrichment baits sufficient for 16 samples; SureSeq Interpret Software 600075 Get a quote
SureSeq Myeloid Panel (96 reactions) Enrichment baits sufficient for 96 samples; SureSeq Interpret Software 600076 Get a quote
SureSeq NGS Library Preparation Kit (16) Bundle of 1 x library preparation kit (16) containing adaptors, PCR primers and enzymes sufficient for 16 samples and 1 x SureSeq NGS Index Kit – Collection A 500070 Get a quote
SureSeq NGS Library Preparation Kit (48)  Bundle of 3 x library preparation kit (16), containing adaptors, PCR primers and enzymes sufficient for 48 samples and 1 x SureSeq NGS Index Kit – Collection B 500073 Get a quote
SureSeq NGS Index Kit - Collection A (16) 16 different indexes, each sufficient for 4 samples [included with SureSeq NGS Library Preparation Kit (16)] 500071 Get a quote
SureSeq NGS Index Kit - Collection B (48) 48 different indexes, each sufficient for 4 samples [included with SureSeq NGS Library Preparation Kit (48)] 500072 Get a quote
Dynabeads™ M270 Streptavidin, 2ml Sample capture beads, sufficient for 20 samples 500080* Get a quote
AMPure® XP beads, 10ml Sample purification beads, sufficient for 16 samples 500081* Get a quote

 

* Only for use with SureSeq NGS panels

For more information about the SureSeq Core MPN Panel or any cancer analysis products contact us at products@ogt.com.

We would like to thank Professor Nick Cross (National Genetics Reference Laboratory - Wessex, UK) for providing the validated research samples.

SureSeq: For research use only; not for use in diagnostic procedures.

Resources

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Application note

Gene list

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