Achieving high quality microarray results

Tuesday 22 April 2014
Product types:
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Oxford Gene Technology (OGT), The Molecular Genetics Company, has released a new application note detailing the technical evaluation of two DNA labelling kits, where OGT’s CytoSure Genomic DNA Labelling Kits were found to be quicker and more accurate than a leading alternative. Achieving efficient and uniform labelling of both test and reference DNA samples is critical for aCGH studies, enhancing data accuracy and interpretation. By ensuring greater accuracy, OGT’s kits vastly improve the identification of breakpoints and even the smallest aberrations, providing robust and invaluable insights into the underlying cause of genetic disorders.   

During the evaluation, performance was judged on two key quality control metrics of the microarray: the derivative log ratio spread (DLRS), which calculates the probe-to-probe ‘noise’ and thereby indicates the uniformity and accuracy of labelling; and the signal-to-noise ratio, which indicates how accurately each ‘spot’ of signal can be detected. The application note details how, using commercially available DNA samples, CytoSure Genomic DNA Labelling Kits consistently generated both a lower DLRS (below 0.2) and higher signal-to-noise ratio (above 100), compared to the alternative supplier. OGT’s kits also undergo stringent quality control monitoring for every reagent batch to ensure reliable the delivery of such high quality results for every experiment.

CytoSure Genomic DNA Labelling Kits utilise heat rather than restriction enzymes to fragment the DNA samples, thereby streamlining the workflow and reducing loss of material. Removing the restriction digest stage saves approximately 2 hours, allowing the whole procedure to be easily completed in a single day.

These evidence-based findings from OGT highlight the superior performance of CytoSure Genomic DNA Labelling Kits, and this conclusion is also supported by extensive customer evaluations. The Cytogenetics Laboratory, Baylor College of Medicine, commented: “Our lab has tried a variety of different labelling methods; and found OGT’s CytoSure Genomic DNA Labelling Kit to give consistently low DLRS values and excellent signal-to-noise ratios.”

To see the data for yourself, download the application note today.

For more information, please visit www.ogt.com.

For further information, please contact:

Oxford Gene Technology, Begbroke Science Park, Begbroke Hill, Woodstock Road, Begbroke, Oxfordshire, OX5 1PF, U.K.

T: +44 (0) 1865 856826 ; F: +44 (0) 1865 848684

E: contact@ogt.com ; W: www.ogt.com ; Twitter: @OxfordGeneTech

Notes for editors:

About Oxford Gene Technology

Oxford Gene Technology (OGT) provides world-class genetics research solutions to leading clinical and academic research institutions. Founded by Professor Sir Edwin Southern, and with customers in over 60 countries worldwide, OGT has a strong reputation and increasing share in the large and growing genomic medicine market. The Company’s Cytocell®, CytoSure™ and Genefficiency™ range of fluorescence in situ hybridisation (FISH), microarray and next generation sequencing (NGS) products and services deliver high-quality genetic analysis, enabling accurate identification and confirmation of the causative variation underlying genetic disease.

For more information on the Company, please visit our website at www.ogt.com

CytoSure™ and Genefficiency™ NGS browser/report: For Research Use Only; Not for Use in Diagnostic Procedures

CytoSure: This product is provided under an agreement between Agilent Technologies, Inc., and OGT. The manufacture, use, sale or import of this product may be subject to one or more of U.S. patents, pending applications, and corresponding international equivalents, owned by Agilent Technologies, Inc. The purchaser has the non-transferable right to use and consume the product for RESEARCH USE ONLY AND NOT for DIAGNOSTICS PROCEDURES. It is not intended for use, and should not be used, for the diagnosis, prevention, monitoring, treatment or alleviation of any disease or condition, or for the investigation of any physiological process, in any identifiable human, or for any other medical purpose.