CytoCell MALT1 Breakapart FISH Probe

Product summary

Technology FISH
Application Solid tumour
Areas of interest Lymphoma
Region 18q21.31-q21.32
Label
Product Code LPS 017 (10 tests)
LPS 017-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

MALT1, 18q21.31-q21.32, Red
MALT1, 18q21.31-q21.32, Green

The MALT1 Breakapart probe consists of two green clones (132kb and 306kb) and two red clones (171kb and 228kb), which are positioned on each side of the MALT1 gene.

Probe information

Extranodal marginal zone B-cell lymphoma (MZBCL) of the Mucosa-Associated Lymphoid Tissue (MALT) type is characterised by two main translocations involving MALT1; t(11;18)(q21;q21.32) and t(14;18)(q32;q21.32)¹. Other MALT1 associated translocations are rare¹.

However, there are no MALT1 translocations in nodal or splenic lymphomas¹. The t(11;18) BIRC3 (Baculoviral IAP repeat containing 3 protein^2,3)/MALT1 translocation is present in 18-33% of cases of MALT lymphoma² and the resulting fusion protein induces NF-κB (nuclear factor kappa-light-chain-enhancer of activated B-cells), resulting in transcriptional activation³. 18% of MALT lymphomas harbour the t(14;18) MALT1/IGH translocation⁴.

The genetic abnormalities in MALT lymphomas reflect their site of origin. The t(14;18) translocation was most frequently found in MALT1 lymphomas of the parotid gland, liver, skin, and ocular adnexa – sites rare for the t(11;18). However, the pulmonary and gastrointestinal MALT lymphomas show high frequencies of t(11;18) and absence of the t(14;18)^1,4.

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.